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1 2025

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Deuterium isotope effects in mechanistic studies of biotransformations of L-tyrosine and p-hydroxyphenylpyruvic acid catalyzed by the enzyme L-phenylalanine dehydrogenase

Pałka Katarzyna, Podsadni Katarzyna, Szymańska-Majchrzak Jolanta, Winnicka Elżbieta

Nukleonika

2025

Vol. 70, No. 2

51--56

The mechanisms of the reversible oxidative deamination of L-tyrosine to p-hydroxyphenylpyruvic acid and reductive amination of phenylpyruvic acid to L-phenylalanine, both catalyzed by the enzyme L-phenylalanine dehydrogenase (PheDH, EC 1.4.1.20), were investigated using the kinetic isotope effect (KIE) and solvent isotope effect (SIE) methods. The values of deuterium kinetic effects in the 2-position of L-tyrosine and KIE in the (3S)-position of phenylpyruvic acid and solvent isotope effects for both reactions were determined using the non- -competitive spectrophotometric method. Some mechanistic details of these biotransformations were discussed.