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Transcriptomic data analysis of melanocytes and melanoma cell lines of LAT transporter genes for precise medicine

Szczepanek Monika, Panek Dominik, Przybyło M., Moskal Paweł, Stępień Ewa Ł.

Bio-Algorithms and Med-Systems

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2022

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Vol. 18, no. 1

144--150

EN

Background: Boron Neutron Capture Therapy (BNCT) is a two-step treatment that can be used in some types of cancers. It involves administering a compound containing boron atoms to the patient and irradiating the affected area of the body with a neutron beam. The success of the therapy depends mainly on the delivery of the boron isotope (10B) to the tumor using an appropriate boron carrier. One of the boron carriers used is boronophenylalanine (BPA). Therefore, in research on the use of boron carriers, it is also important to know the mechanisms of its uptake by cells. Aim: To study the expression of LAT family genes in two melanoma (high melanotic WM115 and low melanotic WM266-4) cell lines and melanocytes (HEMa-Lp) which are responsible for the transport the BPA into cells. Methods: To normalize data from the transcriptomic analysis, the ratio of the median method was used. This allowed the samples to be compared with each other. Comparison metrics included log-fold change (LFC) values. The heatmap of LFC values and the cluster map were created. These graphs show the similarities and differences between the samples. Results: Transcriptomic data show that in melanocytes, LFC for SLC7A5 (LAT1) and SLC3A2 (4Fhc) was higher than in melanoma cell lines, which corresponded with their melanin content. Conclusion: Our results indicate overexpression of BPA transporter genes in normal cells (melanocytes), which may suggest the highest level of these proteins in melanocytes compared to less melanotic melanoma. Therefore, for BNCT, the use of BPA as the 10B carrier will require additional qualifying tests of amino acid transporter expression for patients and specific tumors to develop a personalized BNCT.

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Projekt utworzenia Polskiej Sieci Biobanków - aktualizacja postępu prac oraz zakres produktów oferowanych do wdrożenia w polskich biobankach ludzkiego materiału biologicznego

Kalinowski Leszek, Kozera Łukasz, Skokowski Jarosław

Laboratorium Medyczne

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2019

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nr 4

50--54

PL

Od niespełna trzech lat w Polsce funkcjonuje Konsorcjum BBMRI.pl jako narodowy reprezentant w europejskiej sieci biobanków Biobanking and Biomolecular Resources Research Infrastructure (BBMRI-ERIC.eu), która jest organizacją naukową służącą realizacji badań wykorzystujących materiał biologiczny pochodzący od człowieka do rozwoju medycyny personalizowanej. Konsorcjum BBMRI.pl pracuje nad rozwiązaniami biotechnologicznymi i organizacyjnymi na rzecz utworzenia Polskiej Sieci Biobanków.

EN

The BBMRI.pl consortium has been functioning in Poland for less than three years as a national representative in the European biobanking network Biobanking and Biomolecular Resources Research Infrastructure (BBMRI-ERIC.eu), which is a scientific organization serving the implementation of research using biological material from humans for the development of personalized medicine. The BBMRI.pl consortium is working on biotechnological and organizational solutions for the creation of the Polish Biobanking Network.

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Ethical consequences of current transformation processes of medicine from professional ethics point of view

Kovaľová D.

Zeszyty Naukowe. Organizacja i Zarządzanie / Politechnika Śląska

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2018

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z. 122

109--116

EN

In the paper, we emphasize the idea of radical changes in contemporary medicine that have occurred as a result of the unprecedented development of scientific and technological progress. There is a growing need to analyse the current state and problems in the field of medicine, to consider its new goals and perspectives with an emphasis on the ethical dimension. From a wide range of questions and problems of contemporary medicine, we examine the ethical aspects of the patient's autonomy, commercialization of medicine and personalized medicine. We point out that applied occupational ethics in this field also provides a strategy for cultivating professional responsibility for doctors (healthcare professionals).

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Hiperpolaryzacyjne związki kontrastujące w obrazowaniu MRI : przygotowanie kontrastu

Lorenc K., Nieciecki M., Cacko M., Czarniecki M., Zalewska E., Królicki L.

Inżynier i Fizyk Medyczny

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2015

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Vol. 4, nr 5

245--248

PL

Zastosowanie kontrastów hiperpolaryzacyjnych jest znaczącym krokiem w rozszerzeniu zastosowań MRI w zakresie obrazowania molekularnego. W artykule przedstawiono niezbędne i zalecane wyposażanie pracowni prowadzącej badania z wykorzystaniem kontrastów hiperpolaryzacyjnych, a także proces przygotowania próbki do hiperpolaryzacji.

EN

The application of hyperpolarized contrasts in MRI is a step towards molecular imaging. This article discusses the prerequisites and recommended laboratory apparatus for implementing hyperpolarized contrast agents’ use in diagnostics. The process of sample preparation is described and basic concepts in DNP polarization are introduced.