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Biomimetic alginate/perfluorocarbon microcapsules - tthe effect on in vitro metabolic activity and long-term cell culture

Stefanek Agata, Kulikowska-Darłak Aleksandra, Bogaj Karolina, Nowak Aleksandra, Dembska Joanna, Ciach Tomasz

Chemical and Process Engineering

2022

Vol. 43, nr 1

81-–95

Cell encapsulation seems to be a promising tool in tissue engineering. However, it has been shown to have several limitations in terms of long-term cell cultures due to an insufficient oxygen supply. In this study we propose the use of novel microcapsules designed for long-term cell culture consisting of an alginate shell and perfluorocarbon (PFC) core, which works as a synthetic oxygen carrier and reservoir. The influence of PFC presence in the culture as well as the size of structures on cell metabolism was evaluated during 21-day cultures in normoxia and hypoxia. We showed significant improvement in cell metabolism in groups where cells were encapsulated in hydrogel structures with a PFC core. The cells maintained a typical metabolism (oxidative phosphorylation) through all 21 days of the culture, overcoming the oxygen supply shortage even in large structures (diameter ¡ 1 mm). Applying PFC in alginate matrices can improve cell metabolism and adaptation in long-term cell cultures.

Wpływ uregulowań unijnych dotyczących postępowania z F-gazami na krajowy rynek urządzeń gaśniczych

Zbrożek P., Kiełbasa T.

Bezpieczeństwo i Technika Pożarnicza

2011

Nr 1

105-116

W artykule przedstawiono uregulowania prawne dotyczące F-gazów wykorzystywanych w ochronie przeciwpożarowej. Omówiono stosowanie F-gazów oraz konsekwencje nowych uregulowań dla branży przeciwpożarowej. Zarysowano prognozy rozwoju urządzeń gaśniczych wykorzystujących F-gazy oraz przedstawiono rozwiązania alternatywne.

In the article there are described legal regulations concerning F-gases used in fire protection. F-gases use is discussed together with consequences of new regulations of fire protection industry. Forecasts of development of firefighting devices using F-gases are outlined and alternative solutions are presented.

Experimental Evaluation of the Importance of the Pulmonary Surfactant for Oxygen Transfer Rate in Human Lungs

Sosnowski T. R., Gradoń L., Skoczek M., Droździel H.

International Journal of Occupational Safety and Ergonomics

1998

Vol. 4, No. 4

391--409

The rate of oxygen transport from atmospheric air into water and perfluorocarbon compound (PFC) was investigated. Static and dynamic systems with and without the presence of the lung surfactant monolayer were considered. For the case of water used as an oxygen absorbent, the monolayer activity allowed a simulation of the gas uptake into the lung hypophase. In the second case, a two-phase liquid system with water as a hypophase and PFC as the blood substitute simulated oxygen transport in the alveolus-blood system. Original experimental measurement devices gave the opportunity of determining the gas transport rate with the possibilities of indicating the role of the lung surfactant in the process and evaluating the influence of environmental conditions on the transport phenomena. Results of that work suggest a possible enhancing role of the lung surfactant in the oxygen transfer rate.