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Retention and separation efficiency of some synthetic oligopeptides in reversed-phase thin-layer chromatography

Gwarda R. Ł., Tomczyszyn A., Misicka A., Dzido T. H.

Acta Chromatographica

2015

Vol. 27, no. 1

1--14

Influence of the following variables such as adsorbent type, type and concentration of organic modifier in mobile phase, type and concentration of ion-pairing reagent, or pH of the mobile phase buffer on retention of some synthetic peptides in reversed-phase high-performance thin-layer chromatography systems has been investigated. The investigations have been also focused on influence of the variables mentioned on solute zone shape regarding optimization of their separation. Remarks about solute retention mechanism have been also provided.