Wyniki wyszukiwania - BazTech

1

Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged and uncharged ferroelectric crystal surfaces

Vandrovcova M., Bacakova L., Vanek P., Petzelt J.

Engineering of Biomaterials

|

2016

|

Vol. 19, no. 135

2--7

EN

The cell-material interaction is significantly influenced by the physicochemical properties of the material surface, including its electrical charge. In this study, the effect of the surface polarity of ferroelectric LiNbO3 single crystals on the adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells was investigated. The cells were cultured on the normal-to-plane poled and in-plane poled plates resulting in positive, negative and zero surface charge. The number of initially adhering cells on day 1 after seeding, their spreading, shape, and their metabolic activity, production of type I collagen, activity of alkaline phosphatase and mineralization in the following days of cultivation (days 6 and 20) were comparable on all three tested surfaces. However, significant differences were found in the expression of mRNA for type I collagen, alkaline phosphatase and osteocalcin, i.e. an early, medium-term and late arkers of osteogenic cell differentiation, respectively. On day 20, the expression of type I collagen was significantly lower in cells on negatively-charged than on non-charged surfaces. Moreover, the expression of alkaline phosphatase and osteocalcin was higher in cells on positively-charged than on negatively-charged surfaces. These differences were generally more pronounced in standard cell culture medium than in osteogenic medium, which could, at least partly, mask the influence of the material surface properties on the cell behaviour. Thus, positively-charged LiNbO3 surfaces seemed to be more suitable for the osteogenic differentiation of bone marrow mesenchymal stem cells than the negatively-charged surfaces.

2

Wpływ zabiegów uzdrowiskowych na obrót kostny na podstawie obserwacji grupy pacjentek w 20. Wojskowym Szpitalu Uzdrowiskowo--Rehabilitacyjnym w Krynicy-Zdroju

Mleczko M., Damijan Z., Błaszczyk J.

Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna

|

2007

|

Vol. 13, nr 3

226-229

PL

Celem pracy było określenie wpływu wybranych zabiegów fizjoterapeutycznych na ruchomość kręgosłupa oraz występowanie parestezji u osób ze zmianami zwyrodnieniowymi odcinka szyjnego kręgosłupa leczonych zachowawczo. Po zastosowanym leczeniu fizjoterapeutycznym wszystkie oceniane zakresy ruchomości uległy poprawie, doszło również do zmniejszenia parestezji w obu badanych grupach.

3

Adhesion, growth and differentiation markers in human osteoblast-like cells cultured on surface-modified metallic materials designer for bone implants

Bacakova L., Kabatova J., Lisa V., Stary V., Fencl J.

Inżynieria Biomateriałów

|

2006

|

R. 9, nr 58-60

1-3

EN

A series of metallic materials with different surface treatments were prepared: pure machined titanium (T), titanium polished by diamond paste (TL), machined Ti6Al4V alloy (TS), Ti6Al4V alloy polished by diamond paste (TSL), Ti5Al2.5Fe alloy treated by electro-erosion (A) and Ti5Al2.5Fe plasma-sprayed with Ti (PL). The materials were seeded with human osteoblast-like cells MG 63. One day after seeding, the highest cell numbers were obtained on the samples of medium surface roughness (T and TS; Ra 0.63-0.30 um and 0.89-0.57 um, respectively). From day 1 to 4, the cell proliferation was the quickest on the samples with the lowest surface roughness (TL and TSL; Ra 0.17-0.13 for both materials). The cells on TL also contained the highest concentration of integrin adhesion molecules with alpha V chain, i.e. receptors for vitronectin and fibronectin. One day 8 after seeding, the cell on all metallic samples as well as tissue culture polystyrene reached similar population densities. The cells on electro-eroded Ti5Al2.5Fe (samples A; Ra 15.27-0.74 um) contained the highest concentration of osteocalcin and osteopontin, i.e. markers of osteoblastic differentiation. Thus, the latter newly developed material could be considered as promising for construction of bone implants well anchored in the surrounding bone tissue.