The pyrimido[4,5-e][1,2,4]triazine was prepared as a new ring system via annulation of the triazine ring onto the preformed pyrimidine derivative. Various S-benzoylisothiourea derivatives were synthesized by condensation of benzoylthiocyanate with amines. All the synthesized compounds were tested as antimicrobial agents and compounds 11 and 12 showed significant activity to ward to Klebsiella pneumoniae.
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