Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Acta Chromatographica

Powiadomienia systemowe

Sesja wygasła!

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: ondansetron

Sortuj według:

Ogranicz wyniki do:

A simple HPLC method for the determination of butorphanol tartrate and ondansetron hydrochloride in patient-controlled analgesia solution

Fang B., Wang L., Chen F., Li P., Shi X.

Acta Chromatographica

2018

Vol. 30, no. 1

43--46

A simple and rapid high-performance liquid chromatographic (HPLC) method was established for simultaneous determination of butorphanol tartrate and ondansetron hydrochloride in analgesic mixture samples used for patient-controlled analgesia (PCA). The separation of butorphanol tartrate and ondansetron hydrochloride in PCA solution was carried out on phenomenex C18 column (4.6 mm × 150 mm, 5 μm) using 50 mM sodium acetate (pH 4.0) buffer and acetonitrile (72:28, v/v). Flow rate was 1.0 mL min−1 with a column temperature of 30 °C, and detection wavelength was carried out at 280 nm and 306 nm. Validation of the method was made in terms of specificity, linearity, accuracy, and intra- and inter-day precision, as well as quantification and detection limits. The developed method was successfully used to evaluate the chemical stability of butorphanol tartrate and ondansetron hydrochloride in analgesic mixtures at the usual concentration used for PCA.

Development and validation of an RP-HPLC-UV method for the determination of ondansetron in rabbit plasma: Application to a pharmacokinetic study

Ravi S., Khan N., Darwis Y

Acta Chromatographica

2011

Vol. 23, no. 4

579--593

A new sensitive and specific isocratic RP-HPLC-UV method was developed and validated for the determination of ondansetron in rabbit plasma using risperidone as an internal standard (IS). The sample preparation involved a simple deprotenization procedure with a mixture of 1 mL of acetonitrile and 50 μL of 10% w/υ zinc sulfate. Analysis was performed on a Phenomenex CN column (250 mm × 4.6 mm, 5 μm) with 50 mM ammonium acetate (pH 3.5) and acetonitrile (35:65, υ/υ) as mobile phase at a flow rate of 1.0 mL min-1. Column eluent was monitored at 310 nm. The calibration curve was linear over the concentration range of 25–1000 ng mL-1. (r2 = 0.9999) with a limit of quantification (LOQ) 25 ng mL. -1.The intraday and interday precision and accuracy were between 0.93% and 3.41% and −3.63% and 1.01%, respectively. The mean recoveries of ondansetron and risperidone were 85.87% and 99.80%, respectively. Ondansetroncontaining plasma samples were stable at −20°C for 14 days. The validated method was successfully applied for a pharmacokinetic study after a single oral administration of ondansetron (8 mg) to rabbits.