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EN
The objective of this study was to determine the effects of various concentrations of water extracts prepared from the fresh or dry matter of lemon balm on Sitona lineatus L. and Aphis fabae Scop. behaviour. The assessment pertaining to the feeding intensity of beetles was carried out by measuring the surface of feeds caused by S. lineatus. While examining the effect of extracts on A. fabae, the mortality of wingless female and aphid larvae was determined. In the studies on the olfactory reaction glass olfactometer "Y tube" and 4 armed arena olfactometer were used. The results of the experiment showed that the water extract prepared from dry matter of lemon balm with 2% concentration limited the feeding of both female and male of S. lineatus. The increase in the mortality of the black bean aphid females and larvae was obtained only after applying the extracts from fresh and dry matter at highest concentrations. The evident deterrent reaction of the odour substances obtained from the lemon balm plants towards the beetles of S. lineatus, could find application in ecological farms via introducing the plant as an accompanying crop to the main crops. The winged individuals of A. fabae did not react to the abovementioned factor.
EN
The objective of this study was to determine the effects of various concentrations of water extracts prepared from the fresh or dry mass of lemon balm on the feeding of Colorado potato beetle larvae in L2 and L4 stages. The extracts from the dry matter were prepared at the concentrations of 2%, 5% and 10% and from the fresh matter at the concentrations of 10%, 20% and 30%. The feeding intensity assessment was conducted by dipping leaves of potato in respective solutions of the extracts and determining the mass of food consumed by larvae, as well as the changes of larvae body weight. In the studies on the olfactory reaction of adult insects, a glass “Y-tube” olfactometer was used. The feeding by L4 larvae of Colorado potato beetle was effectively limited by the extract prepared from the dry matter of lemon balm with 10% concentration. The larvae at L2 stage turned out to be more susceptible to the effects of extracts – the extracts in almost all used concentrations limited the feeding of the studied pest. The body weight gain in L2 and L4 larvae of Colorado potato beetle were effectively limited by the extracts prepared from the dry plants of lemon balm in as low concentration as 5%. However, the effect of extracts prepared from the fresh matter of lemon balm plants was effective in limiting the body weight gain of L4 larvae at the minimum concentration of 20%, whereas for L4 larvae the effective concentration was 30%. The evident deterrent reaction of odour substances originating from lemon balm plants towards the females of Colorado potato beetle was found. The males did not react to the above-mentioned factor.
EN
The aim of the experiment was to determine the effect of aqueous extracts of dried sage (Salvia officinalis L.) in concentrations of 2%, 5% and 10%, and the fresh parts of the plant in concentrations of 10%, 20% and 30% on the feeding of Sitona lineatus L. on the broad bean (Vicia faba L.) leaves. The olfactometry response of the pest (response to olfactory stimuli) was also determined in relation to the sage. Extracts of dry and fresh parts of sage reveal the inhibitory effect on feeding of both males and females of pea leaf weevil, the stronger, the higher their concentrations, however, no apparent differences were registered between the analysed sexes. Dry plant extracts in 10% concentrations and 30% extracts of fresh sage parts caused 2–3 time reduction of Sitona lineatus feeding after 4 days of the experiment. An apparent repellent response of aroma substances originating from sage towards weevil beetles (both males and females) was registered, which suggests potential applications of this plant as a neighbouring plant in mixed stands or, e.g., as a component of border belts.
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