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Utlenione pochodne cholesterolu : występowanie, rola biologiczna, metody analizy

Bodzek D., Janoszka B., Wielkoszyński T.

Wiadomości Chemiczne

2000

[Z] 54, 11-12

1089-1108

The trends of cholesterol oxidation in living organisms and the main products of this process have been presented [1-7]. Biological activity of oxycholesterols have been characterised as well as their occurence in biological material [8-32]. A three-step analytical procedure of oxycholesterols determination has been suggested. A range and conditions for the following methods (Solid Phase Extraction, Thin Layer Chromatography, Gas Chromatography, High Performance Liquid Chromatography, Mass Spectrometry) application in oxycholesterols separation, identification and quantitative determination have been discussed [33-63]. Reagents most often used for oxycholesterol derivatization as well as reagents used for oxycholesterol visualization after the separation by TLC method have been presented. Some difficulties in analysing these compounds have been mentioned.