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Point-of-Care Testing : Biosensor for Norepinephrine Determination

Baluta Sylwia, Swist Agnieszka, Cabaj Joanna, Malecha Karol

International Journal of Electronics and Telecommunications

2020

Vol. 66, No. 2

369--372

An useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)- 9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with thin polymeric film. This sensing arrangement utilized the catalytic oxidation of NE to norepinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of enzyme – laccase. With the optimized conditions, the analytical performance demonstrated selectivity in a wide linear range (0.1–200x10-6 M) with a detection limit of 240 nM and a quantification limit of 365 nM. Moreover, the method was successfully applied for selective NE determination in the presence of interfering substances.

Deuterium isotope effects in oxidation of dopamine and norepinephrine catalyzed by horseradish peroxidase

Byszewska W., Pająk M., Kańska M.

Nukleonika

2013

Vol. 58, No. 4

475--479

Deuterium kinetic isotope effects (KIE’s) in the reaction of oxidation of dopamine (DA) catalyzed by the enzyme horseradish peroxidase (HRP, EC 1.11.1.7) was determined using a non-competitive spectrophotometric method. For kinetic studies, the deuterium ring labelled isotopomer [2’,5’,6’-2H3]-dopamine was synthesized using acid catalyzed isotopic exchange between authentic dopamine and heavy water. Deuterium solvent isotope effects (SIE’s) for dopamine and norepinephrine were determined separately for the enzymatic oxidation carried out in the presence or absence of hydrogen peroxide. Some mechanistic details of enzymatic oxidation of dopamine and norepinephrine to corresponding catecholchromes catalyzed by HRP were discussed.