Ultisol is a type of soil with low organic matter, pH, and nutrient content, including molybdenum, leading to low productivity. This study aimed to investigate the use of dry land using molybdenum and lime (CaCO3) inoculated with Rhizobium strain Nod+Fix+ to increase the soybean production of Willis and Baluran cultivars. This research was conducted from May to September 2021 in Pallangga Subdistrict, Gowa Regency, South Sulawesi, Indonesia. The study used a split-plot design with three replications for each treatment. The first factor was soybean varieties, consisting of Baluran and Willis cultivars. The second factor was the composition of the bacterial strain Nod+ Fix+, lime CaCO3 and NH4-molybdate, which consisted of without (Rhizobium strain Nod+ Fix+ + CaCO3 + NH4-molybdate); Rhizobium strain Nod+ Fix+ + CaCO3 1.0 ton/ha + NH4-molybdate 250 g/h); Rhizobium strain Nod+ Fix+ + CaCO3 1.5 tons/ha + NH4-molybdate 500 g/h); and Rhizobium strain Nod+ Fix+ + CaCO3 2.0 tons/ha+ NH4-molybdate 750 g/h). The results showed that treating the bacterial strain Nod+ Fix+ + MoCo (1.0:0.6) kg/ha achieved the best results on growth, nutrient uptake (Nitrogen, Phosphorus and Potassium), and soybean yields, both for Willis and Baluran varieties on ultisol soils.
The aim of this work was to examine the effects of A. chroococcum on the prolifera-tion of R. leguminosarum bv. viciae (Rlv) in a solid-carrier inoculant and on symbiotic effectiveness of Rlv with pea plants grown under laboratory and field conditions. In a laboratory experiment it was found that proliferation of both bacterial species, Rlv and A. chroococcum, in the dual-culture inoculants was efficient, and that A. chroococ-cum had no adverse effects on the development of the rhizobia (Rlv) in the solid-carrier inoculant. In a pot experiment the highest number of nodules was detected on roots of pea plants inoculated with the dual-culture inoculant containing Rlv and A. chroococcum, slightly lower numbers on pea roots inoculated with the mono-culture inoculum of Rlv and almost no nodules were found on the roots of pea un-inoculated (control treatment) with the bacteria. In the micro-plot experiment conducted in the years 2011–2012 pre-sowing inoculation of pea seeds with the mono-culture inocu-lant of Rlv or with the mixed inoculant of Rlv and A. chroococcum slightly increased nodule numbers/plant, pod numbers/plant and seed numbers/pod, as compared to the un-inoculated control, but these differences were not reflected in pea seed yields/m2, which were similar in all treatments.
Celem badań była ocena różnych szczepów bakterii symbiotycznych łubinu i soi pod względem ich oddziaływania na brodawkowanie i plon nasion wymienionych roślin. Do badań użyto izolatów Bradyrhizobium sp. (Lupinus) i Bradyrhizobium japonicum znajdujących się w kolekcji bakterii symbiotycznych Z. Mikrobiologii IUNG-PIB w Puławach, którymi zaszczepiono nasiona łubinu żółtego (odm. Mister) i łubinu wąskolistnego (odm. Baron) oraz soi (odm. Aldana i Nawiko). W doświadczeniu wazonowym (wazony Mitscherlicha z 7 kg gleby) nie stwierdzono na ogół istotnego wpływu zastosowanych szczepów Bradyrhizobium sp. na plonowanie łubinu, chociaż większość użytych szczepów stymulowała brodawkowanie na korzeniach tej rośliny. Badane szczepy B. japonicum różniły się pod względem ich oddziaływania na brodawkowanie soi, ale wszystkie szczepy tych bakterii symbiotycznych przyczyniły się do istotnego wzrostu zarówno intensywności brodawkowania, jak i plonowania obydwu odmian soi.
EN
The aim of these studies was to assess various isolates of symbiotic bacteria of lupine and soybean with respect to their effects on nodulation and seed yields of the crops. Strains of Bradyrhizobium sp. (Lupinus) and Bradyrhizobium japonicum, belonging to the collection of these bacteria at Dept. of Agricultural Microbiology IUNG-PIB Pulawy, were used for inoculation of lupine seeds (cv. Mister and Baron) and soybean seeds (cv. Aldana and Nowiko). In pot experiments (Mitscherlich pots containing 7 kg of soil) none of the tested strain of lupine rhizobia significantly increased lupine seed yields though some of these strains improved nodulation of lupine plants. All B. japonicum strains – soybean symbionts – markedly stimulated nodulation and significantly improved seed yields of both soybean cultivars.
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The aim of the performed experiments was to analyse relationships occurring between endophytic bacteria from the Herbaspirillum genus and Sinorhizobium meliloti Bp nodule bacteria and to examine the condition of plants subjected to coinoculation with the above-mentioned strains in in vitro conditions. In experiments examining the impact of Herbaspirillum frisingense on Sinorhizobium meliloti BP, the stimulation of growth of inoculated bacteria from the Sinorhizobium genus was recorded in all three combinations (48-hour culturing, sediment and supernatant). On the other hand, the examination of interactions between the Sinorhizobium meliloti strain and Herbaspirillum frisingense strain revealed that in the case of culture and supernatant, an antagonistic action was recorded. Besides, it was found that such coinoculation exerted a beneficial influence on the process of seed lucerne symbiosis and yielding as confirmed by increased numbers of root nodules, higher nitrogenase activity and greater plant mass.
PL
Celem doświadczenia była analiza wzajemnych relacji zachodzących pomiędzy bakteriami endofitycznymi z rodzaju Herbaspirillum frisingense i bakteriami brodawkowymi Sinorhizobium meliloti Bp oraz zbadanie kondycji roślin poddanej koinokulacji wyżej wymienionymi szczepami w warunkach in vitro. W badaniu oddziaływania Herbaspirillum frisingense na Sinorhizobium meliloti BP we wszystkich trzech kombinacjach (hodowla 48h, osad i supernatant) zanotowano stymulację wzrostu wysianych bakterii z rodzaju Sinorhizobium. Z kolei badanie oddziaływania pomiędzy szczepem Sinorhizobium meliloti a szczepem Herbaspirillum frisingense wykazało, że w przypadku hodowli i supernatantu zanotowano antagonistyczne oddziaływanie. Na podstawie uzyskanych wyników z przeprowadzonych badań w warunkach laboratoryjnych nad efektem jednoczesnej inokulacji lucerny wyżej omawianymi szczepami bakterii Sinorhizoium meliloti i Herbaspirillum frisingense, stwierdzono korzystne oddziaływanie takiego szczepienia na proces symbiozy i plonowanie lucerny siewnej.
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