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The influence of autologous and heterologous extract of antimicrobial peptides on leukocytes isolated during titanium implant insertion in rabbit and ovine model

Zdziennicka Joanna, Szponder Tomasz, Latalski Michał, Żylińska Beata, Sobczyńska-Rak Aleksandra, Wessely-Szponder Joanna

Engineering of Biomaterials

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2021

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Vol. 24, no. 159

24--30

EN

This study evaluated the in vitro leukocyte response to titanium implants in the presence of autologous or heterologous antimicrobial peptides extracts. Antimicrobial peptides (AMPs) appeared to be a new approach both against microorganisms and for regulation of inflammatory and repair processes. To evaluate their potential usefulness in regenerative medicine, we prepared different extracts of neutrophil-derived AMPs from rabbit, ovine or porcine blood which contained AMPs of different compositions, mainly defensins, cathelicidins and fragments thereof. Then, we assessed in vitro the influence of different AMPs extracts on the neutrophils and monocyte-derived macrophages (MDM) activity. For this purpose, these cells were obtained from experimental animals, rabbits, or sheep submitted to insertion of a titanium implant into the tibial defect. The cultured cells stimulation was autologous or heterologous, dependently on the AMPs extract origin and the experimental animal species. The neutrophil activity was assessed on the basis of the enzymes release from azurophilic and secondary granules and the free radicals generation. The MDM functional assessment was based on the NO and superoxide generation and arginase activity. Additionally, morphological changes were evaluated in the cell cultures. Our results indicated that the origin of AMPs extract is crucial for its activity. The autologous extracts stimulated anti-inflammatory responses, whereas the heterologous extracts displayed pro-inflammatory effect on neutrophils and macrophages. These results might be considered during the introduction of new preparations in regenerative medicine.

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Mathematical modeling of the neutrophil production process supported by administration of glycoprotein

Mika Barbara, Pelka Magdalena, Tkacz Ewaryst

Biocybernetics and Biomedical Engineering

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2021

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Vol. 41, no. 1

45--63

EN

Drugs administered in chemotherapy influence the patient's hematopoietic system. The side effects of chemotherapy can force changes in both the dose of drugs and the schedule of their administration, which in turn may adversely affect the patient's health. During chemotherapy treatment, there is a necessity to continuously monitor basic blood parameters, including the number of neutrophils and blood platelets, responsible for the patient's immunity. The aim of this paper is to study the behavior of the hematopoietic system, during chemotherapy, by means of a modified mathematical model of neutrophil production. We conducted simulations for children from 1–15 years old, suffering from neuroblastoma, who are undergoing chemotherapy treatment with Topotecan (TPT). The proposed model provides an effective tool for signaling the moment when the number of mature neutrophils, circulating in the blood, drops below the critical level, that is, 500 cells/mL. Such a nadir persisting over time may result in severe neutropenia and consequently require a halt or change to the established treatment schedule. In addition, we analyzed the process of neutrophil production during chemotherapy, supported by the application of glycoprotein as a growth factor of stem cells.

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Wpływ proszków węglowych na ludzkie krwinki białe

Bąkowicz-Mitura K., Czerniak-Reczulska M., Baj Z.

Engineering of Biomaterials

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2007

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Vol. 10, no. 63-64

32-34

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Software for cells images segmentation

Strojny W., Korzyńska A.

Journal of Applied Computer Science

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2003

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Vol. 11, nr 1

91-100

EN

The paper contains the description of software for microscopic cells images segmentation called SeKom. Methods of computer images processing based on texture analysis were used for image sequence recorded cell behaviour, segmentation. The programme is a part of the System of Monitoring of Cells Behaviour elaborated and constructed in Institute of Biocybernetics and Biomedical Engineering, PAS for the project of cell movement and behaviour investigation.