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Rapid comparison of antitumor chemical constituents and mechanisms between Dendrobium nobile and Dendrobium officinale by UPLC-IT-TOF, network pharmacology and experimental verification

Liu Jie, Li Zhaoyang, Zeng Meiling, Liang Yan, Liang Bing, Ge Qiuping, Zhang Xiaoyan, Zhang Shilin, Zhou Wei

Acta Chromatographica

2023

Vol. 35, no. 2

123--138

Dendrobium nobile and Dendrobium officinale as the main varieties of traditional Chinese medicine Dendrobium are widely used in clinic. The study aimed to systematically explore chemical constituents and their antitumor effect of D. nobile and D. officinale by ultra-performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UPLC-IT-TOF), network pharmacology and cancer cell experiments. D. nobile extract and D. officinale extract could significantly inhibit the proliferation of human lung cancer A549 cells, human liver cancer HepG2 cells and human breast cancer MCF-7 cells in the dose-dependent manner (P < 0.05), the antitumor effect of D. officinale extract was stronger than that of D. nobile extract at the same drug concentration. A total of 40 chemical constituents of D. nobile and D. officinale including phenanthrenes, bibenzyls and other types of compounds had been identified by UPLC-IT-TOF, LCMSsolution and MetID software according to retention times, accurate mass, MSⁿ fragmentation, reference compounds and natural product databases. Phenanthrenes with good antitumor activity were mainly present in D. nobile, bibenzyls were the main compounds of D. officinale. Integrated networks of Herb-Compounds-Targets-Cancer revealed that gigantol, moscatilin, tristin, moscatin and densiflorol B were regarded as key antitumor compounds of D. nobile and D. officinale, D. nobile and D. officinale shared 7 targets accounting for 70% of the antitumor core targets, more than half of their antitumor KEGG pathways were similar. The results of molecular docking and western blotting experiments indicated that the antitumor mechanisms of D. nobile and D. officinale may be through inhibiting PI3K-Akt and HIF-1α signaling pathways.

Antitumor chemical constituents of Toddalia asiatica (Linn) Lam root bark and its rational alternative medicinal parts by multivariate statistical analysis

Luo Cairong, Liu Jie, Liang Yan, Shen Xiangchun, Zhang Xiaoyan, Zhou Wei

Acta Chromatographica

2021

Vol. 33, no. 2

153--161

Toddalia asiatica (Linn) Lam (T. asiatica) as a traditional Miao medicine was investigated to find rational alternative medicinal parts for T. asiatica root bark and its antitumor chemical constituents by quantitative pharmacognostic microscopy, high performance liquid chromatography (HPLC) fingerprint and multivariate statistical analysis. A bivariate correlation analysis method based on microscopic characteristics and content of chemical constituents was established for the first time, there were some regular discoveries between powder microscopic characteristics and common chromatographic peaks of T. asiatica through quantitative pharmacognostic microscopy, cork cells, calcium oxalate square crystal, brown clump, starch granule and phloem fiber, as powder microscopic characteristics may be placed where the main chemical constitutes were enriched. Scores plot of principal component analysis (PCA) and dendrogram of hierarchical clustering analysis (HCA) showed that 18 T. asiatica samples were distinguished correctly, clustered clearly into two main groups as follows: S01∼S03 (root bark) and S07∼S09 (stem bark) in cluster 1, S04∼S06 and S10∼S18 in cluster 2. Nineteen common peaks were obtained in HPLC fingerprint of T. asiatica, loadings plot of PCA indicated seven compounds played important roles in different part of samples (P10 > P08 > P07 > P14 > P16 > P17 > P19), peaks 04, 06, 07, 08, 10 were identified as hesperidin, 4-methoxycinnamic acid, toddalolactone, isopimpinlline and pimpinellin. MTT assay was used to determine the inhibitory activity of different medicinal parts of T. asiatica on human breast cancer MCF-7 cells, all parts of T. asiatica had different inhibitory effects on MCF-7 cell lines, root and stem barks of T. asiatica showed the best inhibitory activity. The relationship between chemical constituents and the inhibitions on MCF-7 cell had been established, significant antitumor constituents of T. asiatica were identified by correlation analysis, the order of the antitumor effect of the main compounds was P07 (toddalolactone) > P16 > P06 (4-methoxycinnamic acid), P11 > P18 > P10 (pimpinellin) > P08 (isopimpinellin) > P01 > P19 > P14 > P04 (hesperidin) > P17, which were antitumor chemical constituents of T. asiatica root bark. T. asiatica stem bark was the most rational alternative medicinal part for T. asiatica root bark.

O historii leków naturalnych na łamach „Kroniki Farmaceutycznej” w okresie dwudziestolecia międzywojennego (1919 -1939)

Ślusarczyk W.

Kwartalnik Historii Nauki i Techniki

2018

R. 63, nr 1

39--63

In the years 1919-1939, „Kronika Farmaceutyczna” was the press organ of the Trade Union of Pharmaceutical Employees of the Republic of Poland. The magazine was filled with articles devoted to defence of pharmacists’ rights but the aim of its editors was also to publish scientific and popular content, in which a prominent place was occupied by articles on the history of natural medicines. This work attempts to analyse the forms and content of that kind of articles: describe how the subject of old medicines was written about, what types of medicines were discussed, and also who and why wrote about them. Articles devoted to the history of natural medicines can be divided in four categories: scientific and popular articles dedicated to current pharmaceutical issues and preceded by historical introductions; scientific and popular articles on history; summaries from foreign pharmaceutical magazines and news from the world covering pharmaceutical problems of the time, accompanied by historical introductions, as well as summaries from foreign pharmaceutical magazines and historical news from the world. According to our research, the majority of analysed articles belonged to the first category. Articles concerning the past of natural medicines appeared throughout the interwar period and became a permanent element of “Kronika Farmaceutyczna”. They focused on plant drugs, less often on zoonotic and mineral ones. Majority of texts were written according to a pattern based on chronological narration. Historical content was supplemented with ancient mythology information and folk medicine. Abundance of information obtained from contemporary literature dealing with this subject and from the sources proves that authors were undoubtedly erudite people. They committed some mistakes from time to time, though. Their errors, as well as uncomplicated, chronological system of narration were the results of the fact that they were not professional historians but pharmacists. Their interest in the past of natural medicines was to a large extent the outcome of general tendencies predominant in the Polish pharmaceutical milieu at that time. Analysed publications were therefore written by pharmacists and for pharmacists. Their purpose, aside from satisfying readers’ curiosity, was to deepen professional knowledge through enabling the perception of current pharmaceutical problems from the larger, historical perspective.