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Effect of storage time on microwave saturation of EPR spectra of thermally sterilized streptomycin and its practical applications

Ramos P., Pilawa B., Bernaś M.

Journal of Medical Informatics & Technologies

2010

Vol. 15

185--191

Electron paramagnetic resonance spectroscopy at X-band (9.3 GHz) is used as the tool of optimalization of storage of thermally sterilized aminoglycoside antibiotic - streptomycin. Thermal sterilization at different conditions was performed according to the norms. Changes in free radicals system of this drug during storage after sterilization were detected. Lineshape analysis and microwave saturation of EPR spectra were done. Free radical concentrations were determined. Microwave saturation EPR analysis is proposed as useful method to determine the best conditions of storage of sterilized drugs.

Influence of oxygen O2 on microwave saturation of EPR lines of plants carbonized at 650°c and potential application in medicine

Pilawa B., Bartłomiejczyk S., Krzesińska M., Pusz S., Zachariasz J., Wałach W.

Engineering of Biomaterials

2008

Vol. 11, no. 73

9-11

Electron paramagnetic resonance spectroscopy (EPR) was used to examination of spin-lattice relaxation in vascular plants carbonized at 650°C. Application of EPR method of continuous microwave signal saturation in medicine was proposed. The first derivative EPR spectra of pyrolysed bamboo and yucca were measured for samples in air and in vacuum. Influence of microwave power in the range 0.7-100 mW on amplitudes of EPR lines were evaluated. It was stated that amplitudes of the studied carbon materials increase with increasing of microwave power in the studied range. Saturation of EPR lines was not observed, so fast spin-lattice relaxation processes exist in the analyzed materials. Changes of EPR amplitudes for samples in air were slower than for evacuated samples. This effect may be used for determination of oxygen contents in the biological cells cultures and for analysis of optimal parameters of photodynamic therapy of cancer.