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The membrane with polylactide and hyaluronic fibers for skin substitute

Stodolak-Zych E., Rozmus K., Dzierzkowska E., Zych Ł., Kapacz-Kmita A., Gargas M., Kołaczkowska E., Cieniawska M., Książek A., Ścisłowska-Czarnecka A.

Acta of Bioengineering and Biomechanics

2018

Vol. 20, no. 4

91--99

Skin substitutes are heterogeneous group of scaffolds (natural or synthetic) and cells. We hypothesize that nanofibers with layer composition made of polylactide (PLA) and sodium hyaluronate (HA) obtained using electrospinning method are a good matrix for cell adhesion and proliferation. Methods: Optimal conditions of electrospinning of PLA and HA nanofibers to create layered compositions (PLA membrane covered with HA nonwovens) were determined by modifying parameters such as the appropriate amount of solvents, polymer concentration, mixing temperature and electrospinning process conditions. By changing the parameters, it was possible to control the diameter and properties of both polymer fibers. The spinning solution were characterized by surface tension and rheology. A scanning electron microscope (SEM) was used to determine the morphology and fiber diameters: PLA and HA. Structure of the PLA/HA nonwoven was analyzed using spectroscopy (FTIR/ATR). Biocompatibility of the nonwoven with fibroblasts (ECM producers) was assessed in the in vitro conditions. Results: The results showed that stable conditions for the formation of submicron PLA fibers were obtained using a 13% wt. solution of the polymer, dissolved in a 3:1 mixture of DCM:DMF at 45 °C. The hyaluronic fibers were prepared from a 12% wt. solution of the polymer dissolved in a 2:1 mixture of ammonia water and ethyl alcohol. All materials were biocompatible but to a different degree. Conclusions: The proposed laminate scaffold was characterized by a hydrophobic-hydrophilic domain surface with a maintained fiber size of both layers. The material positively underwent biocompatibility testing in contact with fibroblasts.

Growth and osteogenic differentiation of human osteoblast-like cells on nanofibrous scaffolds loaded with diamond nanoparticles: improvement or impairment?

Musilkova J., Stankova L., Potocky S., Kromka A., Stranska D., Bacakova L.

Engineering of Biomaterials

2016

Vol. 19, no. 138 spec. iss.

Preliminary study on the electrospun PLA–based nanofibres as biomaterials for the treatment of cartilage

Magiera A., Markowski J., Lesiuk M., Sieroń A. L, Pilch J., Błażewicz S.

Engineering of Biomaterials

2014

Vol. 17, no. 128-129

110--111

Electrospinning is a simple and universal way to produce fibres from a variety of materials having diameters ranging from submicrometers to nanometers. Such fibres can be formed from resorbable and non-resorbable polymers, ceramics and their different combinations containing nanoparticles. Such a method has gained a great interest in medicine due to its ability to form a fibrous space architecture, similar to the natural extracellular matrix [1,2]. On the other hand, due to a wide range of technical facilities of electrospun fibers the method allows to create directionally-dependent space architecture of nanofibres which mimic natural tissues [3]. Considering the similarities between the microstructure created by nanofibres and the extracellular matrix, nanofibrous materials made by ES technique seem to be promising scaffolds to regenerate cartilage [4] and neural tissue [5]. A material which is used for cartilage scaffolds should mimic native cartilage, which is known to have an oriented structure associated with its mechanical and physiological functions [5]. Scaffold with a biomimetic-oriented architecture is an important requirement for tissue-engineered cartilage. In this study, PLA oriented and non-oriented fibrous scaffolds were manufactured. Selected properties of the materials were analysed and dissussed in view of the manufacture of optimal structure for cartilage tissue engineering.