Wyniki wyszukiwania - BazTech

Ograniczanie wyników

1 Biotechnology and Food Science

1 2016

Znaleziono wyników: 1

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: multipleks PCR

Sortuj według:

Ogranicz wyniki do:

Detection of meat adulteration in veal sausages using a multiplex PCR technique

Sawicki W., Żochowska-Kujawska J.

Biotechnology and Food Science

2016

Vol. 80, nr 1

19--27

Previous methods used to detect veal contamination by lower value meat lacked easiness and accuracy This study describes a simple procedure for detecting origin of meat in processed meat products. A rapid (5h) protocol based on multiplex PCR was developed to detect undeclared chicken meat (a specific mitochondrial DNA sequence) in Polish veal sausages. Simultaneously a PCR assay was successfully optimized for amplification of 274-bp DNA fragment extracted from meat products using designed species-specific primer pairs for the detection of veal meat. This procedure also enabled differentiation of bovine or/and chicken meat from their less expensive porcine substitute.