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1

Pharmacokinetics and bioavailability of curdione in mice by UPLC-MS/MS

Liang Qishun, Chen Tianyu, Luo Lvqi, Ma Yizhe, Wen Congcong, Huang Xueli

Acta Chromatographica

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2023

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Vol. 35, no. 2

144--148

EN

A UPLC-MS/MS method was developed to determinate curdione in the mouse blood, and the pharmacokinetics of curdione in mice after intravenous (5 mg kg⁻¹) and oral (20 mg kg⁻¹) administration were studied. The HSS T3 column was used for separation, and column temperature was set at 40 °C. Multiple reaction monitoring (MRM) mode were used for determination of curdione. Blood samples were taken from the caudal vein of Institute of Cancer Research (ICR) mice after administration of curdione. It showed a good linear relationship in the range of 1–500 ng mL⁻¹ (r > 0.998); the intra-day precision was <13%, the inter-day precision was <15%, and the accuracy was 90%–105%, the recovery was >77%, and the matrix effect was 97%–107%. The half-life was relatively short, and the bioavailability was 6.5%. The developed method was suitable for the pharmacokinetics of curdione in mice.

2

An UPLC-MS/MS method for quantification of spiraeoside in mouse blood and its application to a pharmacokinetic and bioavailability study

Cai Xiaojun, Dai Xiangyi, Li Zhou, Chen Junying, Wang Xianqin, Zhang Meiling

Acta Chromatographica

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2023

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Vol. 35, no. 3

272--277

EN

A simple, rapid, and sensitive method based on UPLC-MS/MS was developed to determine spiraeoside in mouse blood, and was applied to the pharmacokinetics and bioavailability of spiraeoside after mice after intravenous (a dose of 5 mg kg⁻¹) and oral (a dose of 20 mg kg⁻¹) administration. On HSS T3 column set at 40 °C, chromatographic separation was obtained with the mobile phase of acetonitrile and 0.1% formic acid using the gradient elution. Spiraeoside and internal standard (IS) were quantitatively analyzed using multiple reaction monitoring (MRM) mode in electrospray (ESI) positive interface. The MRM mode was monitoring the fragmentation of m/z 465.4→303.1 and m/z 451.3→ 289.2 for spironoside and IS, respectively. The results showed a good linear relationship was in the concentration range of 1–200 ng mL⁻¹ (r > 0.998) and the lower limit of quantification (LLOQ) was 1.0 ng mL⁻¹. The intra- and the inter-day precision (RSD%) of the method was within 14.0%, and the accuracy ranged from 90.0% to 115.0%. The extraction recovery of spriaeoside was better than 63.0%, and the matrix effects were in the range of 86%–98%. It also showed the half-life was short, and the absolute bioavailability was 4.0% in mice. Therefore, the established UPLC-MS/MS method was suitable for the pharmacokinetic and bioavailability study of spiraeoside in mice.

3

Determination of oroxin A, oroxin B, oroxylin A, oroxyloside, chrysin, chrysin 7-O-beta-gentiobioside, and guaijaverin in mouse blood by UPLC-MS/MS and its application to pharmacokinetics

Yu Zheng, Chen Fan, Jin Yinan, Zhou Minyue, Wang Xianqin, Shen Xiuwei

Acta Chromatographica

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2022

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Vol. 34, no. 4

394--402

EN

In this study, a UPLC-MS/MS method was developed to measure the concentrations of the flavonoids oroxin A, oroxin B, oroxylin A, oroxyloside, chrysin, chrysin 7-O-beta-gentiobioside, and guaijaverin in the blank mouse blood, and the method was then used in the measurement of the pharmacokinetics of the compounds in mice. Oroxin A, oroxin B, oroxylin A, oroxyloside, chrysin, chrysin 7-O-beta-gentiobioside, and guaijaverin were administered intravenously at a dose of 5 mg kg⁻¹, and the mouse blood (20 μL) was withdrawn from the caudal vein 0.08333, 0.25, 0.5, 1, 2, 4, 6, 8, and 10 h after administration. The mobile phase used for chromatographic separation by gradient elution was composed of acetonitrile and water (0.1% formic acid). The analytes were detected by operating in electrospray ionization (ESI) positive-ion mode using multiple reactions monitoring (MRM). The intra-day and inter-day accuracy ranged from 86.2 to 109.3%, the intra-day precision was less than 14%, and the inter-day precision was less than 15%. The matrix effect ranged from 85.3 to 111.3%, and the recovery of the analytes after protein precipitation were all above 78.2%. This method had the advantages of high sensitivity, accuracy, and recovery, and it had excellent selectivity, which enabled it to be applied to measuring the pharmacokinetics of the analytes in mice.

4

Determination of eugenitin in mouse blood by ultra-performance liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study

Lin Chongliang, Song Dezhen, Jiang Haodong, Luo Lvqi, Bao Xi, Yu Xiaomin, Ma Jianshe, Wang Xianqin, Jiang Xiajuan

Acta Chromatographica

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2022

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Vol. 34, no. 3

253--257

EN

Eugenitin is a non-volatile chromone derivative which is always found in dried flower buds of Syzygium aromaticum L. (Merr.) & L.M. Perry. Until now, there were no reports about the pharmacokinetics of eugenitin in biological fluids. A UPLC-MS/MS method developed to determine eugenitin in mouse blood. The blood samples were prepared by protein precipitation with acetonitrile. Chrysin (internal standard, IS) and eugenitin were gradient eluted by mobile phase of acetonitrile and water (0.1% formic acid) in a BEH C18 column. The multiple reaction monitoring (MRM) of m/z 221.1→206.0 for eugenitin and m/z 255.1→152.9 for IS with an electrospray ionization (ESI) source was used for quantitative detection. The calibration curve ranged from 0.5 to 500 ng/mL (r > 0.995). The accuracy ranged from 98 to 113%, the precision was less than 12%, and the matrix effect was between 86 and 94%, the recovery was better than 81%. The developed method was successfully used for pharmacokinetics of eugenitin in mice after intravenous (5 mg/kg) and oral (20 mg/kg) administration, and the absolute availability of eugenitin was 12%.

5

Myszeida - od parteru wzwyż

Nawrot Radosław

Zieleń Miejska

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2020

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Nr 2

36--37

PL

Paweł i Gaweł w jednym stali domu, Paweł na górze, a Gaweł na dole. W naszych domach Gawłem są szczury, a Pawłem myszy.

6

Research on the possibility of treating streptococcal infections with hyperbaric oxygenation

Wolański Władysław

Polish Hyperbaric Research

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2020

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nr 1(70)

43--46

EN

The aim of the study was to determine the effect of the application of hyperbaric oxygen therapy on the course of an infection with group A type T-3 hemolytic β streptococcus. Experiments were carried out on Porton white mice and in vitro blood plates. General and local infections with streptococci were induced in animals. The infected animals were treated with hyperbaric oxygenation. The lethal effect of infection was significantly inhibited using hyperbaric oxygenation on the first and second day following the infection.

7

Pharmacokinetics of ligustroflavone in rats and tissue distribution in mice by UPLC–MS/MS

Zhou Quan, Zhang Zhiguang, Geng Peiwu, Huang Bingge, Wang Xianqin, Yu Xiaomin

Acta Chromatographica

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2020

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Vol. 32, no. 2

102--106

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for quantification of ligustroflavone, which was then applied in pharmacokinetics study in rat and tissue distribution in mouse. Twelve male Sprague Dawley rats were used for pharmacokinetics after intravenous (2 or 8 mg/kg) administration of ligustroflavone, six rats for each dose. Twenty-five mice were randomly divided into 5 groups (5 mice for each group, 1 group for each time point) and received 16 mg/kg ligustroflavone via intraperitoneal administration. The linear range of the calibration curve was over 2–2000 ng/mL for ligustroflavone in rat plasma and mouse tissues. The intra-day and inter-day precision expressed in % RSD were less than 14%, and the accuracy was between 88.5% and 108.4%.

8

Pharmacokinetics of ebeiedinone in mouse blood by UPLC–MS/MS

Jin Yongxi, Chen Yuyan, Liu Jiawen, Bao Xi, Zhi Yinghao, Wen Congcong, Zhu Wenzong

Acta Chromatographica

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2020

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Vol. 32, no. 3

194--198

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine ebeiedinone in mouse blood, and the pharmacokinetics of ebeiedinone after intravenous (0.5 mg/kg) and oral (2, 4, and 8 mg/kg) administration was studied. Twenty-four mice were randomly divided into 4 groups, 1 group was for intravenous administration (0.5 mg/kg), and other 3 groups were for oral administration (2, 4, and 8 mg/kg), with 6 rats in each group. Yubeinine was used as an internal standard. Multiple reaction monitoring (MRM) mode was used to quantitatively analyzed ebeiedinone m/z 414.4 → 91.1 and the internal standard m/z 430.4 → 412.3 in the electrospray ionization (ESI) positive interface. In the concentration range of 1–2000 ng/mL, the ebeiedinone in the mouse blood was linear (r2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 15%, and the inter-day precision CV was less than 15%. The accuracy ranged from 85.4% to 114.6%, and the average recovery was higher than 61.3%. The matrix effect was between 87.0% and 106.5%. These data met the pharmacokinetic study requirements of ebeiedinone. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of ebeiedinone in mice. The absolute bioavailability of ebeiedinone was 30.6%.

9

Pharmacokinetics and bioavailability of hapepunine in mice by ultra-performance liquid chromatography–tandem mass spectrometry

Chen Lianguo, Zhang Qingwei, Lin Yijing, Lu Xiaojie, Zhong Zuoquan, Ma Jianshe, Wen Congcong, Ding Cheng

Acta Chromatographica

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2020

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Vol. 32, no. 1

44--48

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was established to determine the hapepunine in mouse blood, and the pharmacokinetics of hapepunine after intravenous (1.0 mg/kg) and intragastric (2.5, 5, and 10 mg/kg) administrations was studied. Delavinone was used as an internal standard. The UPLC ethylene bridged hybrid (BEH) C18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 0.1% formic acid with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring (MRM) mode was used for quantitative analysis of hapepunine in electrospray ionization (ESI) positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. The verification method was established in accordance with the US Food and Drug Administration (FDA) bioanalytical method validation guidelines. In the concentration range of 1–1000 ng/mL, the hapepunine in the mouse blood was linear (r2 > 0.995), and the lower limit of quantification was 1.0 ng/mL. In the mouse blood, the intra-day precision coefficient of variation (CV) was less than 12%, the inter-day precision CV was less than 14%. The accuracy ranged from 91.7% to 109.3%. The average recovery was higher than 76.7%, and the matrix effect was between 86.0% and 106.4%. The UPLC–MS/MS method was sensitive, rapid, and selective and was successfully applied to the pharmacokinetic study of hapepunine in mice. The absolute bioavailability of hapepunine was 22.0%.

10

Tissue coefficient of bioimpedance spectrometry as an index to discriminate different tissues in vivo

Li Ying, Ma Ren, Wang Xin, Jin Jingna, Wang He, Liu Zhipeng, Yin Tao

Biocybernetics and Biomedical Engineering

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2019

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Vol. 39, no. 3

923--936

EN

Bioimpedance indicating cell and tissue condition of the living things is of great importance in impedance spectrum analysis and other related researches, in which measurement of the skin impedance is a tricky problem due to the peculiarity of the stratum corneum. The aim of the study is to develop a method to elucidate the skin impedance in a large frequency range and to find out a biomarker to estimate it. In this article, we introduce a non-destructive method of using both surface and needle electrodes to investigate the electrical properties of the skin including stratum corneum of sixteen anesthetized C57BL/6 mice in vivo. A capacitance model was introduced to bridge the complex Debye's model and the practical three-element model. A new biomarker (tissue coefficient) was introduced to compare the whole skin with the viable skin. The contribution of the viable skin to the impedance of the whole skin can be ignored unless the concerned frequency is much higher than 10 kHz. The CG plot show direct link with the e-s plot, and the tissue coefficient shows significant difference between the whole skin and viable skin and reflects the alpha and beta dispersions. The results suggest that the method of using both surface and needle electrodes to investigate the impedance the whole skin including stratum corneum is practical, and the tissue coefficient is especially suitable for in vivo study, and has great potentials in estimation and discrimination of living tissues, cancer detection, bioelectrical impedance analysis (BIA) and other related fields.

11

Pharmacokinetics of 8-O-acetylharpagide in mouse blood by UPLC–MS/MS

Chen Shanjiang, Huang Miaoling, Yu Zheng, He Jiamin, Huang Binge, Wang Xianqin, Ma Jianshe, Wen Congcong

Acta Chromatographica

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2019

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Vol. 31, no. 3

183--188

EN

8-O-Acetylharpagide is the main active component of the herb Ajuga decumbens, which possesses anti-tumor, anti-virus, and anti-inflammation properties. In this study, ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was used to measure the concentration of 8-O-acetylharpagide in mouse blood, with subsequent investigation of the pharmacokinetics of the drug after intravenous or oral administration. Shanzhiside methyl ester was used as an internal standard, and the acetonitrile precipitation method was used to process the blood samples. Chromatographic separation was achieved using an ultra-performance liquid chromatography ethylene-bridged hybrid (UPLC BEH) column (2.1 mm × 50 mm, 1.7 μm) with a gradient methanol–water mobile phase (containing 0.1% formic acid). The flow rate was 0.4 mL/min, and the elution time was 5.0 min. 8-O-Acetylharpagide was quantitatively measured using electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization. The result indicated that, within the range of 5–500 ng/mL, the linearity of 8-O-acetylharpagide in mouse blood was satisfactory (r > 0.995), and the lower limit of quantification (LLOQ) was 5 ng/mL. Intra-day precision relative standard deviation (RSD) of 8-O-acetylharpagide in blood was lower than 9%, and the inter-day precision RSD was lower than 13%. The accuracy range was between 94.3% and 107.1%, average recovery was higher than 91.3%, and the matrix effect was between 100.8% and 110.8%. This analytical method was sensitive and fast with good selectivity and was successfully applied to perform pharmacokinetic studies of 8-O-acetylharpagide in mice. The bioavailability of 8-O-acetylharpagide was 10.8%, and the analysis of the primary pharmacokinetic parameters after oral and intravenous administration indicated that 8-O-acetylharpagide had a significant first pass effect after oral administration.

12

An artificial vision - based computer interface

Gil A., Márquez M., Chacón E., Ramirez A.

Journal of KONBiN

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2008

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No. 5 (8)

27-34

EN

An application has been developed to assist in using software applications to individuals that have null or limited mobility of their upper limbs. The system uses a Web camera for movement patterns recognition of the user's face. The image analysis allows emulating the basic functions of the computer mouse. The face detection process is carried out through the implementation of an algorithm that uses a cascade sort key of Haar-Like type. The application was developed in C++ to be used on Windows XP platform. Tryout of the application has been performed showing excellent acceptation and short time training requirements.

PL

Opracowano oprogramowanie aplikacyjne pomagające korzystać z oprogramowania komputerowego osobom, które mają zerową lub ograniczoną ruchomość ich górnych kończyn. Taki system wykorzystuje kamerę internetową do rozpoznawania wzorców przemieszczeń ruchów twarzy użytkownika. Analiza obrazów pozwala na emulację podstawowych funkcji myszy komputerowej. Proces detekcji ruchów twarzy jest realizowany poprzez implementację algorytmu wykorzystującego kaskadowy klucz sortowania typu Haara. Oprogramowanie aplikacyjne zostało napisane w języku C++ i może być używane na platformie Windows XP. Zostały przeprowadzone próby oprogramowania, spotkały się one z doskonałym przyjęciem i akceptacją, gdyż używanie tego oprogramowania wymaga jedynie krótkiego treningu.

13

MR Imaging of Mouse Heart in vivo Using a Specialized Probehead and Gradient System

Heinze-Paluchowska S., Skórka T., Drelicharz Ł., Chłopicki S., Jasiński A.

Polish Journal of Chemistry

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2006

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Vol. 80, nr 7

1133-1139

EN

Preliminary results of Magnetic Resonance Imaging (MRI) of cardiac function in mice in vivo, using the homebuilt gradient coils and specialized RF probehead are demonstrated. An unshielded gradient system with inner diameter of 60 mm was designed and constructed for the 4.7T/310 magnet with MARAN DRX console (Resonance Instruments). Dedicated probehead, constructed to fit the gradient system, consists of the RF birdcage coil and the animal handling system. ECG-triggered cine images of eight FVB (wild-type) and four transgenic micewith heart failure (Tg alfa q*44) were acquired at physiological temperature (37 graduate C) with a good quality of multislice images in different phases of the cardiac cycle, as well as a good contrast between myocardium and flowing blood. This technique allowed the calculation of the end-diastolic (EDV) and end-systolic (ESV) volumes of working heart that could be used to monitor the development of heart failure in vivo in Tg alfa q*44 mice.