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1 2005

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New possibilities of light microscopy research resulting from digital recording of images

Szczotok A., Roskosz S.

Materials Science Poland

2005

Vol. 23, No. 2

559--565

New research possibilities related to recent improvements in light microscopy are discussed. New research microscopes are fully automated and motorized. All functions, such as those connected with focusing or stage positioning, can be controlled with a PC. Techniques which combine images recorded at different locations on X, Y and Z axes are presented. The first technique - combining high resolution single images, made in X and Y axes on the surface of a specimen, creates overview images of specimens. This is particularly important in quantitative materialography because the technique produces a significant decrease in the edge effect. Examples of applications of PC controlled X-Y stage and stage management software in the study of a duplex steel, made from Ni-base superalloy and welding are presented. Automatic alignment of images made in applications of this technique in the study of a zinc layer on cast iron and thermal barrier coatings on a heat-resisting alloy are shown.