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The use of modern methods as well as modeling and simulation tools in the design of bioreactors allows for the analysis of the flow phenomena in a short period of time without the need of physical model preparation, and thus for the optimization of existing solutions. The article presents the simulations of the aeration process in an SBR-type bioreactor, realized by means of computational fluid dynamics (CFD) and ANSYS 12.1 software. The subject of the analysis was a diffuser of own design. The Design Modeler 12.1 module was used for the preparation of geometry representing the analyzed design, and the discretization of the continuous domain was carried out with the ANSYS Meshing 12.1 tool. The ANSYS Fluent 6.3 solver was used For model calculations. On the basis of the results obtained from the conducted simulations, it is possible to predict the parameters which will increase efficiency and effectiveness without the need to build a real set of prototype models of aeration systems. The results obtained indicate that an increase in the aeration velocity results in a decrease in the minimum Y-axis velocity for both the mixture and air. The observed differences are caused by the shape of the geometric model and the velocity of the air outlet through the openings, which affects the hydraulic process in the chamber. These processes affect both the amount of oxygen dissolved in the bioreactor and the behavior of the suspension in volume. The turbulence intensity during the aeration process is concerned mainly in the range from 3.9 to 8.7% and is comparable with the average values of turbulence degree obtained by other researchers. The air bubble diameter ranged from 0.3 to 4.5 mm, in the case of aeration velocity 5.68 cm/s, a significant part of the chamber were air bubbles with a diameter of 2.6 to 3.9 mm, i.e. they were not the limit values.
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