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Ethological Changes and Teratogenesis of Model Organisms as an Indicator of Biotesting of the Electromagnetic Radiation Influence

Wójcik Waldemar, Kalizhanova Aliya, Sakun Oksana A., Nykyforov Volodymyr V., Nykyforova Larysa E., Nahorniak Svitlana V., Kistion Volodymyr Ev., Shevchenko Liudmyla S., Yesmakhanova Laura, Junisbekov Mukhtar, Orazbekov Zhaslan, Turgantarova Aigul

Journal of Ecological Engineering

2022

Vol. 23, nr 7

42--49

With the development of information technology, electromagnetic radiation becomes a tangible view of the physical (wave) environmental pollution. Modern scientific research aimed at the components of the elec tromagnetic environment pollution problems mainly involves the anthropocentric approach. There is no procedure for determining the influence of harmful physical factors on biota, in particular in terms of water (Daphnia magna Straus) and air (Drosophila melanogaster L.) environments. A clear system of rationing of maximum permissible levels of electromagnetic radiation, including volume and ecosystems protected areas has not been developed. The article considers the relevant scientific and practical problem of creating a framework for assessing and predicting the negative impact of electromagnetic radiation on the biota related to ethological changes and teratogenesis. The characteristic of all the constituent elements of the system determines the degree of the negative impact of the induction of the magnetic field on the biota: activity, mortality; reproduction; availability, and frequency of Teratology. A method for determining the activity levels of Daphnia and Drosophila total average activity biota was developed and described. The trajectory patterns of Daphnia motion at low activity in the state of stability, with increased activity in the excited state, were created. The results of the research on the negative impact of electromagnetic radiation of industrial frequency on biota were presented. The critical levels of the magnetic field and noise pollution, which cause the depletion and destruction of the test object, the relationship between ethological changes and the occurrence of mutations depending on radiation levels were determined. The biological test objects were proven to minimize the error of the results of determination of electromagnetic effects on the biota, in comparison with the mathematical methods of research.

Larwy Galleria mellonella a badanie patogenności mikroorganizmów

Sztucki D., Książczyk M.

Laboratorium - Przegląd Ogólnopolski

2018

nr 1

23--26

Owad Galleria mellonella wykazuje wiele cech, dzięki którym można go zaklasyfikować, jako organizm modelowy, przede wszystkim posiada mechanizmy odpowiedzi immunologicznej analogiczne do mechanizmów wrodzonych układu immunologicznego kręgowca. Oprócz innych typowych cech organizmu modelowego, Galleria mellonella może być inkubowana w temperaturze 37°C, dzięki czemu stanowi idealny model do badań nad patogennością mikroorganizmów. Użyteczność larw tego owada została poparta wieloma eksperymentami i naukowymi publikacjami.

Insect Galleria mellonella shows many features that make it possible to classify it as a model organism, above all it has mechanisms of the immune response analogous to the innate mechanisms of the immune system of the vertebrates. In addition to other typical features of the model organism, Galleria mellonella can be incubated at 37°C, making it an ideal model for the research on the pathogenicity of microorganisms. The usefulness of the insect’s larvae has been supported by many experiments and scientific publications.