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Cultivation of soapwort plants (Saponaria officinalis L.) in in vitro cultures

Figas Anna, Lefelbajn Weronika

Infrastruktura i Ekologia Terenów Wiejskich

2023

nr I/1

59--69

Saponaria officinalis L. is a medicinal plant from the family Caryophyllaceae. It occurs in most European countries, as well as in North Africa, America and Western Asia. The aim of the study was to find an optimal method of sterilization S. officinalis seeds in order to obtain sterile seedlings and to multiply axillary shoots from nodal explants isolated from them, on MS medium with the addition of growth regulators (RW). In this study, 4 variants of seed sterilization were compared using NaClO at the following concentrations: 1.5% (2), 2% (3) and 2.5% (4) for a period of 11 minutes. The control was a variant in which 70% C2H5OH was used for pre-sterilization for 1 minute (1). The highest percentage of sprouted, seeds and sterile seedlings and the lowest percentage of contamination were obtained in variant (4). As a result of micropropagation of soapwort in in vitro cultures, axillary shoots were formed from nodal explants from axillary buds. The highest percentage of explants with shoots (95%) and callus tissue (48%) and the highest number of shoots from one explant (5.95) were obtained in the second passage on MS medium with the addition of 4 mg.dm-3 BAP and 0.5 mg.dm-3 NAA. Due to the wide possibilities of using soapwort, it is advisable to continue research aimed at developing an optimal and efficient plant regeneration system of this species.

Mikrorozmnażanie roślin rożnika przerośniętego (Silphium perfoliatum L.) w kulturach in vitro

Figas A., Tomaszewska-Sowa M., Lisiak W.

Ekologia i Technika

2010

R. 18, nr 5

237-242

Celem pracy było podjęcie próby mikrorozmnażania rożnika przerośniętego w kulturach in vitro. W doświadczeniu zbadano zdolność kilku wybranych eksplantatów (fragmenty liścieni, ogonka liściowego, wierzchołki pędu) do regeneracji. Badane eksplantaty wykładano na pożywkę MS z dodatkiem regulatorów wzrostu: NAA (kwas naftylooctowy - auksyna) i BAP (6-benzyloami-nopuryna - cytokinina) w różnych kombinacjach stężeń. Najwięcej pędów bocznych rozwijało się z eksplantatów wierzchołkowych pędu na pożywce zawierającej 5 mg l -1BAP i 1mg l-1 NAA.

Cup plant (Silphium perfoliatum L.) is a perennial herb belongs to Asteraceae family, and it is native only to middle and eastern part of USA and Canada. Cup plant was brought to Europe in the 18th century for its ornamental value. Nowadays, interest of this plant grows up because of its useful utilitarian traits. Cup plant may be cultivated as a medical plant, energetic plant, ornamental plant, melliferous plant and reclamation plant. At present, there is no enough seed of cup plants in Poland. The aim of present paper was in vitro micropropagation of cup plant, the regeneration ability of some explants of cup plant (cotyledon fragments, petiole fragments and shoot tips) was studied. Explants were cultured on Murashige i Skoog'a (MS) (1962) [17] medium with different concentration of NAA (1-naphthaleneacetic acid- auxin) and BAP (6-benzyloaminopurine - cytokinin). The lateral shoots were obtained from shoot tips explants cultured on MS medium supplemented with 5 mgdm-3 BAP and l mg drrr3 NAA.