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Green versatile micellar electrokinetic chromatographic method for determination of six antimicrobial and anti-inflammatory drugs in combined dosage forms

Elmansi Heba, El-Awady Mohamed I., Barghash Sona, El-Razeq Sawsan Abd, Belal Fathalla

Acta Chromatographica

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2023

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Vol. 35, no. 3

233--246

EN

A fast reliable micellar electrokinetic methodology was investigated for the concurrent quantitation of six antimicrobial and anti-inflammatory drugs, namely, ciprofloxacin, dexamethasone, metronidazole, ornidazole, spiramycin and tinidazole. The method has the merits of rapidity, precision, and sensitivity. The separation was carried out in less than 7 min by applying a basic background electrolyte consisting of 25 mM disodium tetraborate buffer, pH 9 containing 50 mM SDS at 25 kV using photodiode array detector at 230 and 315 nm. The internal standard used during analysis was cromolyn sodium and validation was carried out following ICH guidelines. The proposed method showed linear response over the range from 0.5 to 10.0 μg mL⁻¹ reaching limits of detection and limits of quantitation in the ranges of 0.09–0.2 μg mL⁻¹ and 0.27–0.6 respectively. The method's greenness was estimated using the GAPI tool where excellent greenness was concluded. Co-formulated or single-ingredient commercial preparations were investigated and the results were statistically evaluated.

2

Prediction of the Retention Factor in Micellar Electrokinetic Chromatography Using Computational Descriptors and an Artificial Neural Network

Jouyban A., Jalilzadeh H., Yeghanli S., Asadpour-Zeynali K.

Polish Journal of Chemistry

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2005

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Vol. 79 / nr 10

1565-1574

EN

An artificial neural network (ANN) method is proposed to calculate retention factor of analytes using structural features computed using HyperChem software. The absolute average relative deviation (AARD) and individual deviation (ID) are calculated as accuracy criteria. The accuracy of the proposed method is compared with that of previously reported least square models. The proposed method was tested on eight experimental data sets and mean + - standard deviation of AARDs for ANN was 10.7+ -2.1 and those of previous models were 48.5+ -20.4 and 130.1+ -79.7, in which the mean differences were statistically significant (p < 0.001). The distribution of IDs sorted in three subgroups, i.e. _ 10, 10-30 and > 30%, shows the superiority of the ANN over the previous models

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Separation of two isomers of benzocarbazoles by ß-cyclodextrin modified micellar electrokinetic capillary chromatography

Shi X., Ma W., Chen D.

Chemia Analityczna

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2001

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Vol. 46, No. 3

433-438

EN

To optimize the separation conditions of the two isomers of benzocarbazoles, micellar electrokinetic capillary electrophoresis (MECC) was employed in this study. In this procedure, a fused silica capillary of 57 cm x 50 um (50 cm to detector) was used. Running buffer was 5.0 mmol 1(-1) aqueous sodium tetraborate solution containing 70 mmol1 (-1) sodium dodecyl sulfate (pH9.16) and 20 mmol 1(-1) p-cyclodextrin. The separation voltage and the pH value of the buffer were optimized. At the optimized separation conditions, the two isomers were separated well within 10 min. It is thus proved that MECC is an effective method for oil chemical exploration.

PL

W celu optymalizacji rozdzielania dwóch izomerów benzokarbazoli zastosowano mice-larną elektrokinetyczną kapilarną chromatografię. Stosowano kapilary z topionego kwarcu o wymiarach 57 cm x 50 urn, przy odległości 50 cm do detektora. Jako bufor zastosowano 5 mmol 1(-1) roztwór wodny tetraboranu sodowego, zawierający 70 mmol 1(-1)dodecylosiarczan sodowy (pH 9,16) i 20 mmol 1(-1) p-cykodekstrynę. Optymalizowano napięcie i wartość pH buforu. W wybranych warunkach rozdzielania oba izomery ulegają dobremu rozdzieleniu w ciągu 10 min co może być wykorzystane przy identyfikacji złóż ropy.

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Electrophoretic behaviour and quantitative analysis of active components 6,7-dehydroroyleanone and taxoquinone extracted from Salvia deserta Schang

Zhao Y., Cao Q., Chen X., Hu Z., Fu B., Chang J.

Chemia Analityczna

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2000

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Vol. 45, No. 2

237-248

EN

This communication demonstrates that micellar electrokinetic chromatography (MEKC) can be convenient for the determination of minor active components 6,7-dehydro-royleanone and taxoquinone in the extract of the traditional Chinese medicinal herb. 6,7-Dehydroroyleanone and taxoquinone can be monitored selectively and sensitively at 254 nm within 8 min in the plant extract, using alfa-cyclodextrin (alfa-CD) as a MEKC modified. The concentration range was 5-500 ug ml-1 for 6,7-dehydroroyleanone and 2.5-750 ug ml-1 for taxoquinone with good linear correlation coefficients 0.9997 and 0.9989, respectively. It was found that 68.2 ug of 6,7-dehydroroyleanone and 12.5 ug of taxoquinone were contained in 1 g of Salvia deserta Schang roots. The inclusion of 6,7-dehydroroyleanone and taxoquinone with alfa-CD in capillary zone electrophoresis (CZE) was studied, and the inclusion constant logKCD were 4.1 and 3.2 for 6,7-dehydroroyleanone and taxoquinone, respectively.

PL

Wykazano, że metoda elektrokinetycznej chromatografii micelarnej (MEKC) może być zastosowana do oznaczania 6,7-dehydrorojleanonu i taksochinonu w ekstraktach surowców roślinnych. Oba biologicznie aktywne związki są ubocznymi składnikami ziół stosowanych w chińskiej medycynie tradycyjnej. Zastosowanie p-cyklodekstryn jako moodyfikatora, pozwala na selektywne oznaczenie obu związków ze znaczną czułością w ciągu 8 min przy 254 nm. Dla 6,7-dehydrorojleanonu i taksochinonu, w zakresie stężeń 5-500 ug ml-1 i 2.5-750 ug ml-1, otrzymano współczynniki korelacji odpowiednio -0.9997 i 0.9989. Stwierdzono, że l g korzeni Sahia deserta Schang zawiera 68.2 ug 6,7-dehydrorojleanonu i 12.5 ug taksochinonu. Za pomocą kapilarnej elektroforezy strefowej zbadano inkluzje obu związków z alfa-cyklodekstryną. Wyznaczono stałe inkluzji logKcD 4.1 i 3.2 odpowiednio dla 6,7-dehydrorojleanonu i taksochinonu.