Wyniki wyszukiwania - BazTech

Ograniczanie wyników

Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: metody przesiewowe

Sortuj według:

Ogranicz wyniki do:

Evaluation of the recombinant cell-based bioassay as a screening method for PCDD/Fs monitoring in fish tissue

Stypula-Trebas S., Warenik M., Gembal M., Malagocki P., Piskorska-Pliszczynska J.

Environmental Biotechnology

2009

Vol. 5, No. 1

32-41

Among 210 congeners only 17 highly toxic, 2,3,7,8-chlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) are of toxicological concern. They exhibit high binding affinity to an intracellular aryl hydrocarbon receptor (AhR), causing harmful effects at exposure levels of thousands of times lower than most environmental toxicants. The Chemically Activated LUciferase gene expression bioassay (CALUX) utilizes recombinant cells that were transfected with a luciferase reporter gene, which responds to dioxin-like compounds with the induction of luciferase in a time-, dose-, AhR-dependent and chemical-specific manner. The bioassay evaluation concerning the European Community (EC) requirements for the PCDD/Fs determination for the official control of foodstuffs was performed on salmon tissue. In order to evaluate the bioassay performance characteristics, recovery range, limit of detection (LOD), limit of quantification (LOQ) and precision were determined. The results revealed that combining a dichloromethane: hexane extraction, an acid silica plus activated carbon clean-up provides reliable, reproducible (CV = 9-20%) measurements with acceptable recovery (78%) and sensitivity at the required ppt range. Due to the low cost and high throughput characteristics of the CALUX assay, food monitoring for PCDD/Fs may benefit from use of this bioassay as a prescreening tool to select and prioritize samples for subsequent analysis by high resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). Although the bioassay may not be able to specify identity of the reactive substances, it may serve as a very useful tool for the evaluation of contamination sources.

Wykorzystanie metod przesiewowych w zarządzaniu zdrowiem w pracy

Horst W. M., Horst W. F.

Zeszyty Naukowe Politechniki Poznańskiej. Organizacja i Zarządzanie

2007

Nr 46

89-96

W artykule przedstawiono wybrane aspekty systemowego zarządzania zdrowiem w pracy. Omówiono kilka wybranych metod przesiewowych. Wyniki badań uzyskanych z zastosowaniem tych metod pozwalają na selekcję wykonywanych przez pracowników zadań. Selekcja ta jest przydatna w kształtowaniu systemu zarządzania ryzykiem ergonomicznym oraz wspomaga podejmowanie decyzji o wyborze metod dalszej analizy ryzyka utraty zdrowia wywołanego sposobem wykonywania pracy.

The paper presents a new approach in health management systems at work, via application screening methods. Investigation's results allow selecting jobs with ergonomics risk factors. It helps management, especially to make up one's mind (take the decision) about the choice of the type of method for further analysis of breakdown risk/hazard due to the way of human performance activity.

Optimisation of carbon fibres made from commercial polyacrylonitrile fibres using the screening design method

Farsani R. E., Raissi S., Shokuhfar A., Sedghi A.

Materials Science Poland

2007

Vol. 25, No. 1

113--120

Stabilization and carbonisation conditions are the main factors influencing the properties of carbon fibres manufactured from polyacrylonitrile (PAN) fibres. In this report, low-cost commercial PAN fibres (textile grade) were used as precursors and the effects of the two mentioned factors on the tensile strength of carbon fibres were evaluated. By using the well-known Plackett-Burman screening method in the design of experiments, the best conditions to produce an economical product were determined. The results showed that this method can improve carbon fibre tensile strength by more than 15.7%.