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Prospects for the application of aptamer based assay platforms in pathogen detection

Banu Kauser, Mondal Bhairab, Rai Bhawana, Monica N., Hanumegowda Raju

Biocybernetics and Biomedical Engineering

2022

Vol. 42, no. 3

934--949

Aptamer-based diagnostics platforms for animal, human, plant and environmental pathogens are gaining importance as they are rapid, user-friendly, sensitive and selective. However, most of the aptamer-based platforms have not yet become commercially available. The increasing number of publications signifies the applications of aptamer-based platform and their potential. Herein, the present review is to describe, a brief overview of the development of various aptamer-based platforms and their applicability for the sensitive detection of pathogens. In this review, several aptamer-based platforms such as Enzyme linked immunosorbent assay (ELISA)-like assay, Apta blot, Apta Polymerase Chain Reaction (PCR), Apta array, Aptamer-based Lateral Flow Assays (LFA), Aptamer based fluorescence assay, Flowcytometry-based assay, Apta affinity chromatography, microfluidics-based platforms, and various aptasensor have been discussed. Most of the platforms are highlighted will encourage researchers to focus on developing pathogen detection platform for various applications.

Selekcja kwasów nukleinowych in vitro

Nawrot B.

Wiadomości Chemiczne

2000

[Z] 54, 7-8

615-636

In vitro selection of nucleic acids (Systematic Evolution of Ligands by Exponential Enrichment - SELEX) is the selection technique which, from the library of randomised or degenerated RNA or DNA molecules, allows the isolation of nucleic acids with expected biochemical properties. Repeated cycles of selection and amplification have been used to isolate sequences of nucleic acids (aptamers) that bind with high affinity specific molecular targets - proteins or low molecular weight ligands. This method allows also to isolate a number of nucleic acids with catalytic activity and thus to generate new ribozymes and deoxyribozymes. SELEX is an useful tool of molecular biology and bioorganic chemistry for developing new therapeutic agents, as well for the insight in function of RNA in molecular evolution and RNA World hypothesis.