PL
 |
EN
Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 9

Liczba wyników na stronie
first rewind previous Strona / 1 next fast forward last
Wyniki wyszukiwania
Wyszukiwano:
w słowach kluczowych:  mesenchymal stem cells
help Sortuj według:

help Ogranicz wyniki do:
first rewind previous Strona / 1 next fast forward last
1
Content available Current status and future prospects of genome-scale metabolic modeling to optimize the use of mesenchymal stem cells in regenerative medicine and biomaterials
Sigurjonsson Olafur E.
Engineering of Biomaterials
|
2020
|
Vol. 23, no. 158 spec. iss.
7
EN
Mesenchymal stem cells are a promising source for externally grown tissue replacements and patient-specific immunomodulatory treatments. This promise has not yet been fulfilled in part due to production scaling issues and the need to maintain the correct phenotype after reimplantation. One aspect of extracorporeal growth that may be manipulated to optimize cell growth and differentiation is metabolism. The metabolism of MSCs changes during and in response to differentiation and immunomodulatory changes. MSC metabolism may be linked to functional differences but how this occurs and influences MSC function remains unclear. Understanding how MSC metabolism relates to cell function is however important as metabolite availability and environmental circumstances in the body may affect the success of implantation. Genome-scale constraint based metabolic modelling can be used as a tool to fill gaps in knowledge of MSC metabolism, acting as a framework to integrate and understand various data types (e.g., genomic, transcriptomic and metabolomic). These approaches have long been used to optimize the growth and productivity of bacterial production systems and are being increasingly used to provide insights into human health research. Production of tissue for implantation using MSCs requires both optimized production of cell mass and the understanding of the patient and phenotype specific metabolic situation. This review considers the current knowledge of MSC metabolism and how it may be optimized along with the current and future uses of genome scale constraint based metabolic modelling to further this aim.
2
Content available Aqueous biological graphene based formulations for ink-jet printing
Dybowska-Sarapuk Ł., Rumiński S., Wróblewski G., Słoma M., Młożniak A., Kalaszczyńska I., Lewandowska-Szumieł M., Jakubowska M.
Polish Journal of Chemical Technology
|
2016
|
Vol. 18, nr 2
46--52
EN
The aim of the study was to produce heterophasic graphene nanoplatelets based formulation designed for ink-jet printing and biomedical applications. The compositions should meet two conditions: should be cytocompatible and have the rheological properties that allow to apply it with ink-jet printing technique. In view of the above conditions, the selection of suspensions components, such as binder, solvent and surfactants was performed. In the first stage of the research the homogeneity of the dispersion of nanoplatelets and their sedimentation behaviour in diverse solutions were tested. Subsequently, the cytotoxicity of each ink on human mesenchymal stem cells was examined using the Alamar Blue Test. At the same time the rheology of the resulting suspensions was tested. As a result of these tests the best ink composition was elaborated: water, polyethylene glycol, graphene nanoplatelets and the surfactant from DuPont company.
3
Content available Novel approach for utilization of poly(α-esters) and mesenchymal stem cells in vascular tissue engineering
Sekula M., Domalik-Pyzik P., Morawska-Chochół A., Bobis-Wozowicz S., Madeja Z., Chłopek J., Zuba-Surma E.
Engineering of Biomaterials
|
2016
|
Vol. 19, no. 138 spec. iss.
23
4
Content available Graphene-based substrates influence biological and functional properties of human umbilical cord-derived mesenchymal stem cells
Sekuła M., Karnas E., Jagiełło J., Noga S., Adamczyk E., Dżwigońska M., Kmiotek K., Baran M., Madeja Z., Lipińska L., Zuba-Surma E.
Engineering of Biomaterials
|
2016
|
Vol. 19, no. 138 spec. iss.
24
5
Content available Adhesion, growth and osteogenic differentiation of human bone marrow mesenchymal stem cells on positively and negatively charged ferroelectric crystal surfaces
Bacakova L., Vandrovcova M., Vanek P., Petzelt J.
Engineering of Biomaterials
|
2016
|
Vol. 19, no. 138 spec. iss.
28
6
Content available remote Ocena wpływu nanocząstek tlenku żelaza na rozmieszczenie mezenchymalnych komórek macierzystych hodowanych w obecności statycznego pola magnetycznego
Marędziak M., Śmieszek A., Basińska K., Kornicka K.
Przemysł Chemiczny
|
2015
|
T. 94, nr 9
1598-1601
PL
Porównano wpływ różnych kombinacji dodatku nanocząstek żelaza i hodowli w obecności statycznego pola magnetycznego na wzrost i dystrybucję mezenchymalych komórek macierzystych. Oceniono również, czy dodatek tlenku żelaza umożliwia przyciąganie komórek wzdłuż przebiegu linii pola magnetycznego.
EN
Fe2O3 nanoparticles were pptd. with NH3 from aq. soln. of Fe(NO3)2, dispersed in UV field and added to a mesenchymal stem cell culture to move the cells in magnetic field. The orientation of the cells in magnetic field was obsd.
7
Content available Krótki przegląd na temat komórek macierzystych
Kmiecik B., Skotny-Krakowian A., Rybak Z.
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
|
2015
|
Vol. 21, nr 1
40--45
PL
Wykorzystanie komórek macierzystych może mieć duży potencjał w medycynie. Stale jednak toczą się debaty, jakie rodzaje komórek macierzystych powinny być w przyszłości wykorzystywane w celach regeneracyjnych. W poniższej pracy dokonano porównania embrionalnych, indukowanych pluripotencjalnych i somatycznych komórek macierzystych. Jednak, by bezpiecznie wykorzystywać komórki macierzyste do regeneracji ciała ludzkiego, należy jeszcze dokładniej poznać mechanizmy ich działania. Z tego powodu Amerykańska Agencja ds. Żywności i Leków (FDA) już w 2006 roku zdecydowała się na opracowanie procedur, które mają zapewnić bezpieczeństwo pacjentom korzystającym z terapii opartych na komórkach macierzystych.
EN
Applications of stem cells has a huge medical potential. However, among scientists there is an ongoing debate, concerning which stem cells should be used in the future for regeneration purposes. This paper presents a comparison of embryonic, induced pluripotent, and somatic stem cells. Gaining a better understanding of the molecular functioning of stem cells will probably lead to more efficient treatment methods for many incurable diseases. For this reason, American agency for Food and Drug Administration (FDA) established some general procedures, which, to some degree, should ensure the safety of experimental stem cell therapies.
8
Content available remote Investigation of human mesenchymal stem cells culture on nanofibrous polyurethane scaffolds and films
Çelebi-Saltık B., Öteyaka M.Ö.
Journal of Achievements in Materials and Manufacturing Engineering
|
2014
|
Vol. 64, nr 2
53--61
EN
Purpose: of this study was to evaluate whether electrospun porous nanofibrous scaffold of polyurethane (PU) with low and high beads accommodate the viability and growth of human bone marrow mesenchymal stem cells (hBM MSCs) in comparison with flat surface (Polypropylen). Design/methodology/approach: To our knowledge, the influence of the beads density on nanofibrous scaffold has never been investigated. For this purpose, we electrospun PU to fabric two porous nanofiber scaffolds with less and high density beads to enhance cells attachment and proliferation of hBM MSCs. Moreover, those surfaces were compared to a flat surface (PP). The samples were studied using scanning electron microscopy (SEM), Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) and static contact angle measurement. Findings: The characterization of the samples revealed that hydrophilic surface of high quantity nanofiber with fewer beads scaffolds (LBNF-PU) had less nanofiber with higher quantity of beads that were overlapped on each other firmly compared to low quantity nanofiber with more beads scaffolds (HBNF-PU). MSCs cell morphology on both HBNF-PU and LBNF-PU nanofibrous scaffolds and flat surface was different; it was observed elongated cell shape for LBNF-PU and flat surface and rounded cell shape for HBNF-PU. Live/dead studies confirmed cell viabilities on flat and nanostructured surfaces. Cells expansion on Polypropylen and nanofibrous scaffolds were increased until 7 days of culture. Research limitations/implications: The randomly nanofiber scaffold limited the growth of human bone marrow mesenchymal stem cells (hBM MSCs). The aligned nanofiber scaffold will be evaluated at next investigation. Originality/value: Nanofibrous scaffold have recently draw attention for potential applications in small vascular replacement. Human bone marrow mesenchymal stem cells (hBM MSCs) growth on porous nanofibrous scaffolds is a promising strategy for tissue engineering. The influence of the beads density on nanofibrous scaffold has never been investigated. For this purpose, we electrospun PU to fabric two porous nanofiber scaffolds with less and high density beads to enhance cells attachment and proliferation of hBM MSCs.
9
Content available remote Wpływ materiałów tlenkowych syntezowanych metodą zol-żel na adhezję mezenchymalnych komórek macierzystych
Marycz K., Krzak-Roś J., Śmieszek A., Grzesiak J., Donesz-Sikorska A.
Przemysł Chemiczny
|
2013
|
T. 92, nr 6
1097--1100
first rewind previous Strona / 1 next fast forward last
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.