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EN
High-performance liquid chromatography (HPLC) is a widely used technique for the simultaneous detection and quantification of different drugs. The purpose of the current study was to develop a simple and cost-effective reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of tizanidine (TZN) HCl and meloxicam (MLX) in rabbit's plasma. Assay of TZN and MLX was performed after extraction of drug from plasma by liquid–liquid extraction technique using methanol and diethyl ether as protein precipitants. Isocratic elution was performed in a Kromasil® C18 column (dimension, 250 × 4.60 mm; particle size, 5 μm) with mobile phase consisting of methanol–water (8:2). Orthophosphoric acid was used to adjust the pH of the mobile phase 3.0, and detection was done at 228 nm. Flow rate was 0.8 mL/min with ambient temperature and average operating pressure of 1400 psig. Retention time of TZN was 2.612 min and that of MLX was 6.960 min with a resolution of 3.18. Both drugs showed satisfactory linearity in the range of 10 to 50 ng/mL with correlation coefficients (R2) of 0.9989 and 0.9972 for TZN and MLX, respectively. The developed method was validated successfully for linearity, system suitability, intra-day and inter-day accuracy, and precision, robustness, and specificity following International Conference on Harmonization (ICH) guidelines. Conclusively, a precise, stable, reproducible, economical, and suitable method for estimation of pharmacokinetic evaluation was developed and validated.
EN
In the present study, we have developed and validated an analytical method for the determination of meloxicam in liposomes using high-performance liquid chromatography-ultraviolet (HPLC-UV). Chromatographic separation was carried out on an Ascentis RP amide C16 column selecting a mobile phase composed of acetonitrile-0.3% formic acid solution (40:60, v/v) adjusted at pH 2.8. The mobile phase flow rate selected was 0.5 mL min -1 and UV detection at 355 nm. Piroxicam was chosen as internal standard. All the analyses were performed at temperatures of 40.0 ± 0.5°C. The calibration curve was linear over the range 18–420 ng mL -1. Relative standard deviation (RSD) for precision was <1.03%. Accuracy ranged between 98.53% and 101.41% with RSD lower than 1.5%. LOD and LOQ were 5 ng mL -1 and 15 ng mL -1, respectively. The method was simple, rapid, and easy to apply, making it very suitable for routine analysis of meloxicam in liposomes. The method could also be used with reliability for the determination of meloxicam in other pharmaceutical dosage forms.
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