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1

Application of atomic force microscopy in analysis of material surfaces

Konstantová V.

Zeszyty Naukowe Politechniki Świętokrzyskiej. Nauki Techniczne

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2011

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Z. 16

47-55

EN

Atomic Force Microscopy (AFM) is from the family of Scanning Probe microscopy common used technique for imaging analysis of material. This paper describe fundamentals of Atomic Force Microscopy - contact, semicontact and noncontact mode. Topography by semicontact mode will be presented by scan images of different type of materials, biological and technical. This work was done with the financial support of the VEGA project n. 1/0564/10 "Research of Structures, Morphologies of Surface and Properties of Nature Materials as Source of Inspiration for Non-conventional constructional Materials".

2

Protein conformational changes induced by adsorption onto material surfaces: an important issue for biomedical applications of material science

Ballet T., Boulange L., Brechet Y., Bruckert F., Weidenhaupt M.

Bulletin of the Polish Academy of Sciences. Technical Sciences

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2010

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Vol. 58, nr 2

303-315

EN

Protein adsorption on solid surfaces is a widespread phenomenon of large biological and biotechnological significance. Conformational changes are likely to accompany protein adsorption, but are difficult to evidence directly. Nevertheless they have important consequences, since the partial unfolding of protein domains can expose hitherto hidden amino acids. This remodeling of the protein surface can trigger the activation of molecular complexes such as the blood coagulation cascade or the innate immune complement system. In the case of extracellular matrix, it can also change the way cells interact with the material surfaces and result in modified cell behavior. In this review, we present direct and indirect evidences that support the view that some proteins change their conformation upon adsorption. We also show that both physical and chemical methods are needed to study the extent and kinetics of protein conformational changes. In particular, AFM techniques and cryo-electron microscopy provide useful and complementary information. We then review the chemical and topological features of both proteins and material surfaces in relation with protein adsorption. Mutating key amino acids in proteins changes their stability and this is related to material-induced conformational changes, as shown for instance with insulin. In addition, combinatorial methods should provide valuable information about peptide or antibody adsorption on well-defined material surfaces. These techniques could be combined with molecular modeling methods to decipher the rules governing conformational changes associated with protein adsorption.

3

Elastic electroconducting surface in magnetostatic field

Rudnicki M.

Journal of Theoretical and Applied Mechanics

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2005

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Vol. 43 nr 1

111--118

EN

The dynamical linear theory of a material surface placed in vacuum and subjected to an external strong magnetostatic field is considered. Motion of the surface is described by a position function The material of the surface is assumed to be an isotropic elastic non-magnetizable electric conductor. The residual stress is taken into account. Displacement-based field equations are obtained in coordinate-free notation.

PL

Przedmiotem rozważań jest teoria liniowa powierzchni materialnej umieszczonej w próżni i poddanej działaniu silnego zewnętrznego pola magnetostatycznego. Ruch powierzchni opisuje funkcja położenia. Założono, że materiał powierzchni jest izotropowy, sprężysty, niemagnetozywalny i przewodzący prąd elektryczny. Uwzględniono naprężenia rezydualne. Otrzymano równania rozwiązujące z użyciem przemieszczeń.