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Poprzetoczeniowa choroba przeszczep przeciwko gospodarzowi (TA-GvHD)

Lachert Elżbieta

Laboratorium Medyczne

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2019

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nr 2

25--29

PL

Choroba przeszczep przeciwko gospodarzowi (TA-GvHD) jest dość często występującą niepożądaną reakcją u biorców allogenicznych przeszczepów komórek macierzystych. Warunkiem jej wystąpienia jest przetoczenie immunokompetentnych limfocytów do organizmu biorcy, którego system immunologiczny jest niezdolny do ich zniszczenia lub dawca jest homozygotą pod względem jednego z haplotypów układu HLA biorcy. Powoduje to, że przetoczone limfocyty T nie są niszczone przez sprawny układ odpornościowy, ponieważ traktowane są jako własne. TA-GvHD charakteryzuje ostry zespół objawów klinicznych takich jak: objawy ze strony układu pokarmowego, zmiany skórne oraz upośledzenie funkcji szpiku (pancytopenia). Czynniki ryzyka leżące u podstaw rozwoju TA-GvHD nie są w pełni zdefiniowane, ale zaobserwowano je u osób z wrodzonym lub nabytym niedoborem odporności, przetoczeniami składników krwi pobranych od krewnych, transfuzją wewnątrzmaciczną i transfuzjami płytek krwi dobranymi w układzie HLA. Przetoczenia wszystkich komórkowych składników krwi takich, jak krwinki czerwone, krwinki płytkowe i koncentraty granulocytarne mogą wpływać na TA-GvHD.

EN

Transfusion-associated graft-versus-host disease (TA-GvHD) is quite a common adverse reaction observed after the transplantation of allogenic stem cells. It occurs when a recipient’s immune system is unable to recognize and destroy the transfused T-lymphocytes or when the donor is homozygous for one of the haplotypes of the recipient HLA system. The transfused T lymphocytes are not destroyed by the efficient immune system of the recipient because they are treated as its own. TA-GvHD is an acute clinical syndrome which involves damage to gastrointestinal tract, skin and bone marrow (pancytopenia). The risk factors underlying the development of TA-GvHD are not fully defined, but it is commonly seen in individuals with congenital or acquired immunodeficiency, transfusions from blood relatives, intrauterine transfusion and HLA-matched platelet transfusions. Transfusions of all cellular blood components, including red cells, platelet and granulocyte concentrates, may implicate TA-GvHD.

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Effect of different lymphocyte-bacteria ratios and bacterial toxins on apoptosis of bovine mammary gland lymphocytes

Slama P., Sladek Z., Rysanek D.

Proceedings of ECOpole

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2011

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Vol. 5, No. 1

109--113

EN

Staphylococcus aureus and Streptococcus uberis delay apoptosis of bovine mammary gland lymphocytes following intramammary infusion and in in vitro studies with lymphocyte-bacteria ratio 1:1. In this study, we investigated the effect of different lymphocyte-bacteria ratios on apoptosis of bovine mammary gland lymphocytes in vitro. We found out that lymphocyte-bacteria (S. aureus or S. uberis) ratios 1:10, 1:50 and 1:100 have different effect on apoptosis of lymphocytes than ratio 1:1. Lymphocyte apoptosis was induced 6 hours following incubation with S. aureus or S. uberis with mentioned ratios (1:10, 1:50 and 1:100). In our previous preliminary experiments focused on exploration of chemical components of bacteria on apoptosis of lymphocytes, we established the effect of muramyl dipeptide and lipopolysaccharide on lymphocyte apoptosis only in vitro. Therefore, in the second part of the present study we focused our experiments on investigation of the effect of Gram-negative bacterial toxin lipopolysaccharide on apoptosis of bovine mammary gland lymphocytes in vivo. The results of these experiments suggest that lipopolysaccharide induces apoptosis of lymphocytes following intramammary application. These data need next exploration to reveal detail effects of bacteria or bacterial toxins on lymphocyte programmed cell death in connection with inflammatory process.

3

The effect of particles, including nanoparticles, on macrophages in vitro and in vivo

Revell P.A., Altaf H., McFarlane T., Bociaga D., Mitura K.

Inżynieria Biomateriałów

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2006

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R. 9, nr 56-57

29-31

EN

Macrophages remove foreign material from the body and are recruited to sites were there are particles present. Multinucleate giant cells from by the fusion of macrophages. In the presence of particles, macrophages produce various chemical mediators, known as cytokines, as well as enzymes. Some of the cytokines are pro-inflammatory (for example, IL1Beta, IL6 and TNFalpha) while others promote giant cell formation (GM-CSF, M-CSF, TGF). The presence of these cellular products can be shown by examining tissue sections with immunohistochemistry and by western blotting. The message (mRNA) for the synthesis of these molecules can be demonstrated by in situ hybridization and the polymerase chain reaction. Macrophages process ingested material and present it as antigen to lymphocytes. Antigen-presenting macrophages play an important part in the initiation of metal sensitization. Surface receptors and their counterligands are expressed on macrophages and lymphocytes during antigen presentation. The particles present in tissues around joint prostheses have been isolated and characterized. Over 95% of these are less than 1 micron (ECD) in size. Transmission electron microscopy has revealed nanoparticles of metal in the range 15-20 nm. Such particles are too small to be phagocytosed. Hydroxyapatite, diamond-like carbon (nano-diamond) and metal particles are being studied and results compared with those of particles in the micrometer range. There is a different response to different nanoparticles.

4

Wpływ zmiennego pola magnetycznego na proliferację komórek limfocytarnych in vitro

Roman A., Zyss T., Nalepa I.

Przegląd Elektrotechniczny

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2005

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R. 81, nr 12

25-27

PL

Podjęliśmy próbę wyjaśnienia, w jaki sposób silne impulsowe pole magnetyczne oddziałuje na aktywność proliferacyjną izolowanych limfocytów na stymulacje mitogenami. Wykazano, że ekspozycja limfocytów na pole magnetyczne znacząco hamowała ich aktywność proliferacyjną.

EN

We aimed to find out how the exposure of isolated lymphocytes to a pulsed magnetic field (MF) affected their in vitro proliferative response to mitogenic stimulation. We found that the exposure of lymphocytes to the MFprofoundly inhibited their proliferative response to mitogens.