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Laser generated gold nanoparticles for mass spectrometry of low molecular weight compounds

Płaza-Altamer Aneta, Kołodziej Artur, Nizioł Joanna, Ruman Tomasz

Chemical Technology & Biotechnology

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2022

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Vol. 3

61--69

PL

Preparation of gold nanoparticles (AuNPs) by pulsed fiber laser (PFL) laser generated nanomaterial (LGN) with the use of 2D galvo-scanner (2D GS) is described. The procedure of covering of custom-made stainless steel MALDI targets containing studied objects via nebulization is also presented. Examples of application of new method (PFL-2D GS LGN and nebulization) in laser desorption/ionization mass spectrometry (LDI MS) analyses are shown. These include tests with amino acids and also low molecular weight polymer.

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Grzyby wielkoowocnikowe jako źródło substancji bioaktywnych

Jasicka-Misiak Izabela

Wiadomości Chemiczne

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2020

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[Z] 74, 1-2

71--87

EN

From ancient times medicine and natural products have been closely linked through the use of traditional medicines and natural toxins. Macrofungi have an established history of use especially in traditional oriental medicine. Nowadays, mushrooms comprise a vast and yet largely untapped source of powerful new pharmaceutical products. In particular, and most importantly for modern medicine, they are an unlimited source of compounds that exhibit multidirectional activity: antioxidant, immuno-enhancing, immunostimulatory, antibacterial, antiviral, anti- inflammatory, antidiabetic, antiallergenic and anticarcinogenic [1, 2, 5-8]. In the presented work, a substances with biological activity from mainly Basidiomycota were reviewed, including low-molecular-weight (terpenoids, polyphenols steroids, quinones, amines, cerebrosides) (Tab.1) and high-molecular- weight compounds (homo and heteroglucans, glycans, glycoproteins, glycopeptides, peptides, proteins) (Tab. 2). Selected species of toxic and hallucinogenic mushrooms were also included in the review. At present, mushrooms are considered to be functional food. Beneficial health effects may be achieved through the direct consumption of fruiting bodies or the use of dietary supplements in the form of ready-to-use preparations containing mushroom extracts. The last years is also a period of a flourishing new field of medicine - mycopharmacology. The scientific approach to compounds contained in mushrooms allowed the isolation and identification of many valuable active substances which are used in the prevention and treatment of lifestyle diseases.

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Zastosowanie nanocząstek srebra w laserowej spektrometrii mas oraz w obrazowaniu MS : przegląd

Sekuła J., Nizioł J., Ruman T.

Wiadomości Chemiczne

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2016

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[Z] 70, 7-8

519--539

EN

Metallic nanoparticles, especially silver nanoparticles, have attracted much attention due to their unique physical, chemical and opto-electronic properties. Silver nanoparticles have been successfully applied as a matrix replacement for the laser desorption/ionization time-of-flight mass spectrometry (LDI-ToF-MS). Silver nanoparticles (AgNPs) can efficiently absorb ultraviolet laser radiation, transfer energy to the analyte and promote analyte desorption, but also constitute a source of silver ions suitable for analyte cationisation. Nanoparticles, producing spectra with highly reduced chemical background in the low m/z region, are perfectly suited for low-molecular weight compound analysis and imaging. AgNPs have been demonstrated to allow efficient capture of different chemical compounds (including amino acids, cholesterol, fatty acids) on their surface, thus efficiently promoting their desorption and gas phase cationisation. The minimum detectable amount for those organic and biological molecules is often in the fmol range [23]. Despite the fact that scientists have developed a variety of methods for the synthesis of silver nanoparticles, there are still problems with obtaining surfaces with nanoparticles of high durability and chemical purity. Recently, a successful application of cationic silver nanoparticles (AgNPs), which were placed on MALDI targets for highly sensitive detection of d-ribose at attomolar levels as well as analysis of biological samples such as urine and blood serum [51] was shown. The application of new 109AgNPET surface has been presented with examples of analysis of nucleosides and nucleic bases [60]. One of the main directions of development of LDI-MS is the imaging mass spectrometry (MSI), enabling the visualization of surface distribution of biological samples. The critical limitations of the spatial resolution of MALDI-MSI are the size of the organic matrix crystals and the analyte migration during the matrix application process. To overcome these problems, researchers tried to use nanoparticles as substitutes of organic matrices. In 2013 Ruman group presented that direct contact of the analysed object with Ag nanoparticle-covered target permits direct surface transfer of chemical compounds. The active surface becomes then a “chemical photograph” of an object and allows MS analysis and MS imaging [68].