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Rapid analysis of loratadine in human serum by high-performance liquid chromatography with fluorescence detection

Plenis A., Konieczna L., Olędzka I, Kowalski P.

Acta Chromatographica

2010

Vol. 22, no. 1

69--79

A simple and rapid high-performance liquid chromatographic method with fluorescence detection for analysis of loratadine (LOR) in small volumes of human serum has been developed and validated. After solid-phase extraction (SPE), with thioridazine hydrochloride as internal standard, chromatographic separation was performed on a C 18 analytical column with 70:30 ( v / v ) acetonitrile-water, adjusted to pH 2.7 with orthophosphoric acid as mobile phase at a flow-rate of 1 min mL -1. The column was maintained at 28°C. Fluorescence detection was performed at excitation and emission wavelengths of 265 of 454 nm, respectively. The method was validated for accuracy, precision, selectivity, linearity, recovery, and stability. Absolute recovery of LOR was >93.0%. The limits of detection (LOD) and quantification (LOQ) were 0.07 and 0.2 ng mL -1,respectively. Linearity was confirmed in the range 0.2–30 ng mL -1 (correlation coefficient >0.9998). This HPLC method is selective, robust, and specific and would enable efficient analysis of large numbers of serum samples in support of pharmacokinetic, bioavailability, or bioequivalence studies after therapeutic doses of LOR.

Rapid determination of loratadine in medicine and bio-fluids using a myoglobin-luminol chemiluminescence system

Wang Z., He X., Wu S., Song Z.

Chemia Analityczna

2009

Vol. 54, No. 3

471-481

A sensitive chemiluminescence method for determination of loratadine has been presented. The method is based on the inhibitory effect of loratadine on the chemiluminescence reaction between luminol and myoglobin. The decrement in chemiluminescence intensity changed linearly with the logarithm of loratadine concentration over the range 0.1 -100.0 ng mL-1(R-1 = 0.9992) with a detection limit of 0.03 ng L-1(3&sigma). At a flow rate of 2.0 mL min-1, the complete analytical process could be performed within 30 s including sampling and washing, with a relative standard deviation of less than 3.0% (n = 7). Without any pretreat-ment the proposed procedure was applied to the determination of loratadine in tablets, human serum, and urine samples with recoveries of 91.7-108.3%, 90.1-103.3%, and 94.6-107.6%, respectively.

Przedstawiono czułą chemiluminescencyjną metodę oznaczania loratadyny. W metodzie wykorzystano hamujące działanie loratadyny na chemiluminescencję powstającą podczas reakcji między luminolem i mioglobiną. Natężenie chemiluminescencji zmniejszało się liniowo wraz ze wzrostem logarytmu stężenia loratadyny w zakresie 0,1-100 ng mL2 (R2 = 0,9992), a granica wykrywalności wynosiła 0.03 ng mL2 (3σ). Przy przepływie 2,0 mL min~' kompletne oznaczenie mogło być wykonane w ciągu 30 s (włączając próbkowanie i przemywanie), przy czym względne odchylenia standardowe wynosiło mniej niż 3% (n = 7). Metodę zastosowano do oznaczania loratadyny w tabletkach, we krwi ludzkiej i w moczu, bez wstępnego przygotowania; odzysk wynosił odpowiednio: 91,7-108,3%, 90, l-l 03,3% oraz 94,6-107,6%.