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EN
The timing of harvesting the first regrowth affects the quantity and quality of the harvested forage, induding fibre content Plant fibre is the building block of cell walls, ils components are cellulose, hemicellulose, lignin, undecomposed protein, pectin, water and ash. Its properties are intluenced by factors such as meteorological condilions in a given season, soil conditions and ferlilizalion, as well as the developmentał stage of the plants at the time of harvest. This paper presents the results of a three-year (2014-2016) study conducted on a three-cut permanent hay-meadow located on mineral soil. Mineral fertilization was carried out annually at the following rates: 60 kg·ha-1 N, 30 kg-ha-1 P and 60 kg-ha-1 K. Biomass sampies for testing were taken each year on five dates: April 28-30, May 5-7, May 11-14, May 18-21 and May 24-28. The content of ADF (acid detergent fibre), NDF (neutral detergent fibre), ADL (acid detergent lignin) was determined in the collected plant samples. Next, the effect of the dale of harvesting the first regrowth of the swardon the degree of lignification, dry matter digestibility (DDM), theoretical dry matter intake (DMI) and the relative feed value of the forage expressed by the RFV index was evaluated. The study found an effecel of the timing of harvesting the first regrowth of the sward on the parameters studied. The fibre fraction contents of NDF, ADF and ADL increased each year with the following dates, having an effect on reducing the relative feed value of plants.
EN
During the years 2014-2015, herbage samples of red clover (Trifolium pratense L.) variety Chlumecky and birdsfoot trefoil (Lotus corniculatus L.) variety Leo were harvested at weekly intervals on 9 occasions from late April to late June. Effects of legume plant species and date of harvest on crude protein (CP), water soluble carbohydrates (WSC), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin (ADL) and dry matter digestibility (DDM) were studied. Regardless of the date of harvest birdsfoot trefoil contained more crude protein, NDF and ADF fraction than red clover. Red clover contained more WSC. Crude protein concentration and DDM declined and WSC, NDF, ADF and ADL increased with advancing maturity of both legume species. But the rate of NDF increase with maturity in red clover was slower.
PL
W latach 2014-2015, co tydzień, dziewięciokrotnie, od końca kwietnia do końca czerwca, pobierano próbki roślin dwóch gatunków: koniczyny łąkowej (Trifolium pratense L.) odmiany Chlumecky i komonicy zwyczajnej (Lotus corniculatus L.) odmiany Leo. Badano wpływ gatunku rośliny bobowatej i terminu zbioru na zawartość białka ogólnego (CP), cukrów rozpuszczalnych (WSC), neutralnego włókna detergentowego (NDF), kwaśnego włókna detergentowego (ADF), lignin (ADL) i strawność suchej masy (DDM). Niezależnie od terminu zbioru komonica zwyczajna zawierała więcej białka ogólnego oraz frakcji włókna NDF i ADF niż koniczyna łąkowa. Koniczyna łąkowa zawierała więcej cukrów rozpuszczalnych. Wraz z postępem faz rozwojowych obu gatunków roślin zawartość białka ogólnego i strawność suchej masy malała, a zawartość WSC, NDF, ADF i ADL rosła. Jednak tempo wzrostu zawartości NDF w koniczynie łąkowej było mniejsze.
EN
The aim of this study was to investigate the lignin composition and structure during Napier grass growth. Napier grass showed an increase in lignin, xylan, glucan content and a decrease in acetone extractives and ash content with its growth, while the content of cellulose and holocellulose reached the highest value after four months growth. In addition, milled-wood lignins (MWL) isolated from Napier grass were characterized with 2D-NMR and Py-GC-MS. The results obtained showed that GSH-type substructures were included in Napier grass lignin, whereas the S-G ratio decreased and the abundance of H-substructures increased during lignification. H-units were deposited at the earlier stages, whereas Napier grass was enriched in G-lignin during the late lignification, and S-units were reduced at the final stage. The differences in deposition of lignin units influenced the distribution of the different linkages of lignin units during Napier grass growth. All lignin samples showed the most abundant β-O-4', β-β' resinol linkages, whereas the data obtained by 2D-NMR indicated the similar development in GSH-type substructures with by Py-GC-MS during all stages of lignification.
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