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Effect of the vapour phase on the TLC separation of tropane alkaloids

Gadzikowska M., Jóźwiak G. W., Waksmundzka-Hajnos M.

Acta Chromatographica

2010

Vol. 22, no. 4

515--525

The effect of conditioning the silica layer by mobile phase vapour or ammonia vapour on the retention of alkaloids eluted with non-aqueous mobile phases was examined. The effect of vapour phase composition on system efficiency, peak symmetry, and separation selectivity were also investigated. The most effective and selective systems were used for separation of the alkaloid fraction of Datura innoxia.