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Artificial extracellular matrices of collagen fibrils and lactoferrin as coatings to enhance osteoblast behavior

Vandorovcova M., Bacakova L., Dubruel P., Douglas T. E. L.

Engineering of Biomaterials

2012

Vol. 15, no. 116-117 spec. iss.

132--134

Lactoferrin, a glycoprotein found in milk, has stimulated osteoblast proliferation and differentiation, but has remained relatively unexplored as a biomaterial component. In this study, artificial extracellular matrices consisting of fibrils of collagen type I containing lactoferrin were used as coatings for the biocompatible polymer poly(lactic-co-glycolic acid) (PLGA). The numbers of cells, their viability and proliferation rate were evaluated in various time intervals. Additionally, cell initial spreading area on day 1 was measured. The results show that lactoferrin accelerates fibril-logenesis, leads to increased osteoblast cell numbers 1 and 3 days post-seeding, and encourages their proliferation in each of the tested time intervals.

Structural analysis of the lactoferrin iron binding pockets

Marchewka D., Roterman I., Strus M., Śpiewak K., Majka G.

Bio-Algorithms and Med-Systems

2012

Vol. 8, no. 4

351--359

Lactoferrin (Lf) - member of the transferrin family of proteins responsible for many different functions in the body of mammals participates in regulation of free iron level in the body fluids making the protein bacteriostatic. The main goal of studies was to test the suitability of molecular dynamic simulation to study structural changes in the tertiary structure of lactoferrin. According to ConSurf Server analysis one of the most conservative amino acids was found not only in iron- but also in carbohydrates- binding pockets which may suggest a significant impact of carbohydrates on the functions performed by lactoferrin. Pocket-Finder program applied to find iron-binding pockets revealed the potential Fe binding area. The stability of the ligand deprived protein was verified performing the 50 ns dynamic simulation using the Gromacs program. The tertiary structure changes during the simulation were observed in N-lob solely. No structural changes were observed in C-lob iron-binding pocket.

Effects of supersonic treatment on lactoferrin separation from whey waste using reversed micellar system

Inoue H., Yamasaki M., Lakshmi D. S., Kojo T., Yamagishi S. I.

Environment Protection Engineering

2005

Vol. 31, nr 3-4

5-12

A cationic oleic acid reversed micellar system was used to simplify and enhance the purification of bovine lactoferrin (Lfn) from whey. The rate of Lfn extraction by the application of 30 kHz supersonic treatment to the organic solvent phase (P < 0.01) was by 1.5 time higher compared to non-supersonic treatment. An increase in water content and the light scatter were observed in the organic phase when the supersonic treatment was applied. An increase in the efficiency of Lfn extraction by supersonic treatment of an organic phase was attributable to increased water dispersion in the organic solvent phase and an increase in the orientation of the cationic oleic acid towards microparticles of water in organic solvent phase.