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HPLC analysis of mycolic acids in evaluation of drug susceptibility of Mycobacterium tuberculosis strains - comparison with conventional methods

Walkiewicz R., Chazan R., Grubek-Jaworska H.

Biocybernetics and Biomedical Engineering

2010

Vol. 30, no. 2

3-14

The commonly used phenotypic tests of M. tuberculosis drug susceptibility evaluate drug effects on quantity of colony forming units (CFU) on solid media or CO2 secretion/O2 con-sumption by cultures in liquid media. These tests are not precise enough and need a long time for the growth of the bacilli, so new more accurate and rapid methods are highly needed. The aim of this study was to determine the utility of quantitative HPLC analysis of mycolic acids for drug susceptibility of M. tuberculosis. In 119/120 (99.16%) of the performed tests the HPLC methods showed excellent agreement with the conventional phenotypic tests. The quantitative HPLC analysis of mycolic acids is a fairly exact indicator of tubercle bacilli growth and may be used as a quick and reliable measure of their drug sensitivity.

Computerized "mycobacterium" type recognition using numerical data base of mycolic acids elution profiles

Glapiński J., Walkiewicz R., Safianowska A., Grubek-Jaworska H.

Biocybernetics and Biomedical Engineering

2004

Vol. 24, no. 1

25-38

The aim of this study was to create a standarized numerical data base of high pressure liquid chromatography elution (HPLC) profiles of mycolic acids extracted from the "Mycobacterium" cell wall, with 28 mycobacterial American Type Culture Collection (ATCC) strains taken as a reference for the study. The final result of our work is a notebook of standarized elution profiles and a computer program (MYCAM) for highly effective and specific automatic "Mycobacterium" species identification.