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Simultaneous determination of gallic acid, protocatechuic acid, catechin, epicatechin, quercetin and kaempferol in Chinese chestnut (Castanea mollissima blume) kernel by high-performance liquid chromatography with diode array detection

Wang T., Yang Y. D., Du B., Yu S., Hou W. L.

Acta Chromatographica

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2014

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Vol. 26, no. 3

539--550

EN

Chestnut exhibits anti-inflammatory, styptic, anti-diarrhea, and analgestic effects as a traditional Chinese medicine. There is increasing evidence that shows that the consumption of chestnuts has become more important in human nutrition due to the health benefits provided by the antioxidants. The phenolic compounds are responsible for major bioactivities, such as anti-tumor and anti-oxidation. A high-performance liquid chromatography (HPLC) method with diode array detection (DAD) was established for the simultaneous determination of six phenolic compounds (gallic acid, GA; protocatechuic acid, PR; catechin, CA; epicatechin, EP; quercetin, QU; kaempferol, KA) in Chinese chestnut (Castanea mollissima blume) kernel. The sample followed by separation on Eclipse XDB-C18 column (150 × 4.6 mm, id., 5 μm) with gradient elution of methanol-1.0% acetate acid solution as a mobile phase, at a temperature of 30°C, under the ratio of 1.2 mL min-1, with 5 μL injection volume, and multi-wavelength synthesis was used with DAD. The correlation coefficients were larger than 0.999, the recoveries were 97.58% for GA, 100.41% for PA, 96.23% for CA, 101.38% for QU, 99.15% for EP, and 98.60% for KA, relative standard deviation (RSD) were 1.04% for GA, 1.21% for PA, 1.09% for CA, 1.19% for QU, 1.06% for EP, and 1.20% for KA. This method was applied for the determination of phenolics in chestnut kernel and was found to be fast, sensitive, and suitable.

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Detection and quantification of Naringenin and kaempferol in Melastoma decemfidum extracts by GC-FID and GC-MS

Sarju N, Abd Samad A., Abd Ghani M, Ahmad F.

Acta Chromatographica

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2012

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Vol. 24, no. 2

221--228

EN

Melastoma decemfidum is a plant species from the Melastomataceae family. The plant was reported to have bioactive flavonoids, which showed antioxidant and cytotoxic activities. Extracts from the leaves of 26 plants were made at room temperature with methanol. Detection and quantification of two of the flavonoids, namely naringenin and kaempferol, in the extracts were carried out by using gas chromatography-flame ion detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). By optimizing the key experimental parameters, a linear response for the individual target compounds was obtained in the concentration range LOQ from 3.44 to 8.26 g mL -1 (r2 = 0.9731–0.9772), with LODs from 1.13 up to 2.72 μg mL-1 per 1.0 g of crushed leaves, and with repeatability within the relative standard deviation (RSD) of 1.65–1.81%.

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Validated HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula Linn.

Bhope S. G, Kuber V. V, Nagore D. H

Acta Chromatographica

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2010

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Vol. 22, no. 3

481--489

EN

This paper describes a simple, precise, and accurate HPTLC method for simultaneous quantification of sennoside A, sennoside B, and kaempferol in Cassia fistula whole plant extract. Chromatographic separation of the sample extract was performed on aluminium foil plates coated with silica gel 60 F 254 as stationary phase. The mobile phase was toluene-ethyl acetate-methanol-formic acid 8:10:5:2 ( υ/υ ). Densitometric evaluation of the separated bands was performed at 270 nm. Sennosides A and B and kaempferol were satisfactorily resolved at R F 0.22 ± 0.05, 0.19 ± 0.05, and 0.81 ± 0.05, respectively. Recovery of sennosides A and B and kaempferol from Cassia fistula extract was 98.03, 98.74, and 99.08%, respectively. The method was validated for specificity, accuracy, linearity (100–400 ng per band), and precision (instrument precision in the range 1.03–1.33 and method precision in the range of 1.31–1.75) in accordance with ICH guidelines.

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Simultaneous HPLC-DAD analysis of five flavonoids in diabetic rat plasma and its application in the study of pharmacokinetics

Tang D, Yin Y, Zhang Z., Gao Y., Wei Y, Chen Y, Han Y.

Acta Chromatographica

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2009

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Vol. 21, no. 3

483--497

EN

A HPLC-DAD method for simultaneous analysis of five flavonoids (rutin, quercitrin, quercetin, kaempferol, and isorhamnetin) in diabetic rat plasma has been developed and validated. Separation of the five flavonoids was accomplished on a C 18 column (250 mm × 4.6 mm i.d., 5-µm particle) and detection was performed at 350 nm. The best resolution was achieved with a methanol-0.1% formic acid gradient at a flow rate of 1.0 mL min −1 . The correlation coefficients for all the calibration plots ( r > 0.999) showed linearity was good over the range tested. The relative standard deviation of the method was less than 7% and 10% for intra- and inter-day assays, and average recovery was between 77.2 and 99.2%. Sensitivity was high and detection limits were between 0.006 and 0.02 µg mL −1 . The method has been successfully used to determine drug concentrations in diabetic rat plasma samples and the pharmacokinetics of the drugs.

5

Improved high-performance liquid chromatography-DAD method for the simultaneous analysis of quercetin and kaempferol in the stems of Cissus quadrangularis Linn

Thakur A. K., Jain V., Hingorani L., Laddha K. S.

Acta Chromatographica

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2009

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Vol. 21, no. 1

95-103

EN

A simple, rapid, and specific reversed-phase HPLC method with DAD detection has been used for analysis of two flavonoids, quercetin and kaempferol, in Cissus quadrangularis Linn. The flavonoids were well resolved within 10 min, and quantification was achieved, on an endcapped C 18 column at 370 nm with acetonitrile-phosphate buffer (pH 3.4, adjusted with glacial acetic acid) 60:40 (v/v) as isocratic mobile phase at a flow rate of 1.0 mL min -1. The method was validated for limit of detection, limit of quantification, linearity, precision, accuracy, and recovery. Linearity was demonstrated over the range 0.75 to 10 µg mL -1 for quercetin and 1.0 to 10 µg mL -1 for kaempferol with good correlation coefficients ( r 2 > 0.998). Detection limits were 0.075 and 0.10 µg mL -1 for quercetin and kaempferol respectively. Recovery was 98.0-105.3% for quercetin and 95.0-101.1% for kaempferol. The method was successfully applied to analysis of two flavonoids in four samples of Cissus quadrangularis Linn.