1Hand 13CNMRspectra of isoguanine (2-hydroxyadenine) and 8-oxoadenine inH2Oand DMSO-d6 solutions of various acidity have been interpreted using arguments based on the results of several 1D and 2D NMR experiments and theoretical DFT-based calculations. The collected data for H2O solutions can serve for identification of these compounds in body fluids during medical analysis. The 1H and 13C NMR spectra of the investigated compounds in DMSO-d6 solutions have provided information on their tautomeric forms and the mobility of their exchangeable protons.
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