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Combined pH/Organic Modifier Double Gradient Reversed-Phase HPLC

Kaliszan R., Markuszewski M., Wiczling P.

Polish Journal of Chemistry

2004

Vol. 78 / nr 8

1047-1056

A new procedure of a combined pH and organic solvent double gradient reversed-phase high-performance liquid chromatography (RP HPLC) has been proposed. The procedure is technically feasible and theoretically proved. The method is demonstrated to outstandingly enhance separation of ionizable analytes. The approach consists in simultaneous developing of linear gradients of pH and of the organic modifier in the mobile phase. An extraordinary, systematically increasing eluting power of the mobile phase leads to a remarkable analyte peak compression, accompanied by a strongly reduced peak tailing.