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Optimization of enzyme immobilization on interpolymer membranes

Poźniak G., Turkiewicz B., Rucka M., Zboińska E.

Environment Protection Engineering

2005

Vol. 31, nr 3-4

221-228

The paper presents some preliminary results of assessing the efficiency of papain immobilization on amine modified polyethylene/poly(styrene-co-divinylbenzene) and polyethylene/poly- (vinylbenzyl chloride-co-divinylbenzene) membranes. The enzyme was immobilized due to adsorption. Three process variables were analyzed: concentration of papain in solution, time and temperature of sorption. In order to find the optimal values of these variables, statistical procedure was applied. It was shown that temperature insignificantly affected immobilization and its role could be accepted. The values of concentration and the time that allowed obtaining the most active preparations were also established.