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Determination of γ-aminobutyric acid in leaf, stem and root bark of mulberry by high-performance anion exchange chromatography-integrated pulsed amperometric detection

Kwon H.-J., Kim S. H., Hwang J.-H., Park Y. D.

Acta Chromatographica

2013

Vol. 25, no. 3

581--588

A simple high-performance anion-exchange chromatography-integrated pulsed amperometric detection method was developed to determine the gammaaminobutyric acid (GABA) content in mulberry. GABA was separated in anion-exchange column under isocratic condition of 20 mM sodium hydroxide solution and detected at integrated pulsed amperometric detection at pico-gram levels. The contents of GABA in the leaf, stem, and root bark of mulberry were calculated without any derivatization or sample purification process using this method. This method showed good linearity, precision, and accuracy for GABA analysis.