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TLC–Densitometry analysis of indole compounds in mycelial culture of Imleria badia and Agaricus bisporus enriched with precursors — serine or anthranilic acid

Opoka W., Kała K., Krężałek R., Sułkowska-Ziaja K., Maślanka A., Muszyńska B.

Acta Chromatographica

2018

Vol. 30, no. 4

236--242

Agaricus bisporus and Imleria in vitro cultures were cultivated on modified Oddoux medium, and Oddoux medium was enriched with serine or anthranilic acid. Serine or anthranilic acid was used at the concentrations of 0.1, 0.25, 0.5, and 0.75 g/L of medium. Determination of indole compounds in the obtained biomass was carried out using thin-layer chromatography (TLC) with densitometric detection. In every analyzed sample, presence of serine or anthranilic acid was studied. Comparison of the results obtained for the treatment and control samples allowed us to determine the optimum concentration of serine or anthranilic acid in the medium in order to obtain biomass with increased content of indole compounds. A. bisporus with addition of anthranilic acid or serine to the medium at the concentration of 0.5 g/L was the most beneficial. In the case of Imleria badia, anthranilic acid at the concentration of 0.5 g/L was the most optimal. This is the first report demonstrating the content of indole derivatives in biomass affected by their precursors (serine or anthranilic acid). The study indicates that modification of the medium can provide satisfactory results, and it is worth to search for its new, improved compositions.

Analysis of Indole Compounds Quantity in Biomass of Edible Basidiomycota Species from in Vitro Cultures and from Cultures Supplemented with L-Tryptophan

Muszyńska B., Sułkowska-Ziaja K., Maślanka A., Rojowski J., Opoka W., Łojewski M.

Acta Chromatographica

2016

Vol. 28, no. 2

223--238

The analysis demonstrated that biomass of Agaricus bisporus, Boletus badius, and Cantharellus cibarius contains non-hallucinogenic indole compounds. Addition of l-tryptophan to the in vitro cultures raised the total content of indole compounds. l-Tryptophan became metabolized, causing an increase of the concentration of some indole compounds. The compounds found in the tested biomass from in vitro culture on Oddoux medium without and with addition of l-tryptophan were l-tryptophan, 5-hydroxy-l-tryptophan, serotonin, melatonin, tryptamine, and 5-methyltryptamine (ranged from 4.28 to 132.51 mg/100 g dry weight). l-Tryptophan is an amino acid exogenous to the human body, and therefore, it must be supplied to the body with food. The highest amount of 5-hydroxy-l-tryptophan was found in the extracts from biomass of B. badius cultured on medium with addition of l-tryptophan (132.51 mg/100 g dry weight). Also, in this case, the highest total content of examined indole compounds (168.00 mg/100 g dry weight) was determined. Melatonin was found only in biomass of A. bisporus cultured on medium with addition of l-tryptophan but in smaller amount (4.28 mg/100 g dry weight).