Wyniki wyszukiwania - BazTech

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Analiza możliwości zastosowania półprzewodnikowych źródeł światła w laboratoriach kultur tkankowych - ‘in vitro’

Puternicki A., Lisak E., Tomczuk P.

Przegląd Elektrotechniczny

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2013

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R. 89, nr 9

236-239

PL

W artykule przedstawiono modelowy system doświetlania mikrosadzonek LED DAPLON-Max-Kolor jako alternatywę dla powszechnie stosowanych systemów świetlówkowych, stosowanych w fitotronach roślinnych laboratoriów kultur tkankowych. Opisano wykonane pomiary spektrofotometryczne. Dokonano analizy wyników, ze szczególnym uwzględnieniem kryterium oceny energooszczędności.

EN

The paper describes model system called LED DAPLON-Max-Kolor designed to be applied for illuminating of seedlings in ‘in vitro’ technology. It seems to be an interesting alternative for fluorescent lamps, commonly used for this purposes. The relevant spectro-photometric measurements are described. Discussion of results is stated with special consideration of energy efficiency criteria.

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Potentiometric detection of the metabolic activity of human tumor cells

Kwapiszewska K., Stępień K., Sendys P., Chudy M., Brzózka Z.

Challenges of Modern Technology

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2013

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Vol. 4, no. 1

41--46

EN

Monitoring of cellular viability is a key part of toxicological assays in vitro. On-line monitoring of metabolic activity would be particularly useful for evaluation of responses to potential therapeutic compounds. Current assays are mostly based on fluorescent dyes and optical detection methods. These methods offer high sensitivity and specificity, however are not suitable for long-term on-line observations. Electrochemical methods can be an alternative for current protocols. Electrochemical detection is low cost and label-free, therefore suitable for long-term cell culture monitoring. In this work investigations on human cancer cells viability will be presented. Cells were cultured as two-dimensional monolayer or three-dimensional spheroids. Different cell culture media were examined. Potentiometric detection was used for continuous monitoring of cell culture as well as end-point investigations. Different growth phases were identified using applied method. Finally, response to an anticancer drug was successfully observed.

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Novel designs and technologies for cell engineering

Ziółkowska K., Chudy M., Dybko A., Brzózka Z.

Challenges of Modern Technology

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2011

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Vol. 2, no. 4

54--60

EN

Microfluidic devices, such as lab-on-a-chip systems, are highly advantageous for cell engineering and cell based assays. It is a particularly useful approach for development of the in vitro cellular systems mimicking the in vivo environment. In this paper, a novel lab-on-a-chip device for three-dimensional human cell culture and anticancer drug testing is presented. Cells were cultured as Multicellular Tumor Spheroids (MCTS) — the best cancer tumor model developed so far. Diff erent designs were tested and novel technique of microfabrication in poly(dimethylsiloxane) was developed. MCTS were cultured in a system of polymeric microwells, with the network of microfluidic channels for culture medium flow. Design included optimal shear stress and proper nutrients supply for cultured cells. Final design provided MCTS culture for four weeks with the homeostasis-like state achievement, which is characteristic for the in vivo situation.