Wyniki wyszukiwania - BazTech

1

Predicting immunogenicity in murine hosts with use of Random Forest classifier

Marciniak Anna, Tarczewska Martyna, Kloska Sylwester

Zeszyty Naukowe. Telekomunikacja i Elektronika / Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy

|

2020

|

nr 24

31--43

EN

Biomedical data are difficult to interpret due to their large amount. One of the solutions to cope with this problem is to use machine learning. Machine learning can be used to capture previously unnoticed dependencies. The authors performed random forest classifier with entropy and Gini index criteria on immunogenicity data. Input data consisted of 3 columns: epitope (8-11 amino acids long peptide), major histocompatibility complex (MHC) and immune response. Presented model can predict the immune response based on epitope-MHC complex. Achieved results had accuracy of 84% for entropy and 83% for Gini index. The results are not fully satisfying but are a fair start for more complexed experiments and could be used as an indicator for further research.

PL

Dane biomedyczne są trudne do interpretacji ze względu na ich dużą ilość. Jednym z rozwiązań radzenia sobie z tym problemem jest wykorzystanie uczenia maszynowego. Techniki te umożliwiają wychwycenie wcześniej niezauważonych zależności. W artykule przedstawiono wykorzystanie klasyfikatora Random Forest z kryterium entropii i indeksem Gini na danych dotyczących immunogenności. Dane wejściowe składają się z 3 kolumn: epitop (peptyd o długości 8-11 aminokwasów), główny kompleks zgodności tkankowej (MHC) i odpowiedź immunologiczna. Zaprezentowany model przewiduje odpowiedź immunologiczną na podstawie kompleksu epitop-MHC. Uzyskane wyniki osiągnęły dokładność na poziomie 84% (entropia) i 83% (indeks Gini). Wyniki nie są w pełni satysfakcjonujące, ale stanowią dobry początek dla bardziej złożonych eksperymentów i wyznacznik do dalszych badań.

2

Cyclic Analogue of Human Heat Shock Protein 70(29-42) Fragment. Synthesis, Conformational Studies and Evaluation of Its Immunogenicity

Karawajczyk B., Wirkus-Romanowska I., Wysocki J., Rolka K., Maćkiewicz Z., Głośnicka R., Kupryszewski G.

Polish Journal of Chemistry

|

2001

|

Vol. 75, nr 2

265-273

EN

The cyclic hexadecapeptide containing human heat shock protein 70(29-42) fragment cyclized by the disulfide bridge between two L-cysteine residues introduced at the N- and C-termini was synthesized by the solid phase method. It was established that the cyclic analogue, contrary to its linear counterpart, had much lower ability to generate immune response in rabbits. Conformational studies of cyclic peptide performed using 1D and 2D 1H-NMR spectroscopy in conjunction with theoretical conformational analysis revealed that the cyclization constrained the 3D structure of this peptide, reflected by the observed rate of cis/trans isomerization of Arg9-Thr10 peptide bond and the presence of Gly7-Asn8 peptide bond in cis geometry.We, therefore, postulate that the conformational flexibility in the case of Human Heat Shock Protein fragments is a key element for their immunogenicity.

3

Synthesis of human heat shock protein 70 (29-42) fragment and evaluation of its immunogenicity

Karawajczyk B., Wysocki J., Kunikowska D., Maćkiewicz Z., Głośnicka R., Korzeniowski A., Górski J., Kupryszewski G.

Polish Journal of Chemistry

|

1998

|

Vol. 72, nr 6

1017-1020

EN

The 29-42 fragment of human heat shock protein 70 (hsp 70) was synthesized by the solid phase method. As determined by enzyme-linked immunosorbent assay (ELISA), the synthetic hsp 70(29-42) peptide generated relatively strong immune response in immunized rabbits. Antibody titers were comparable with anti hsp 70 antibody serum level that was induced by immunization with recombinant protein (hsp 70). It was established that antibodies directed against hsp 70(29-42) peptide could be applied in ELISA for detecting hsp 70 in body fluids and tissues.

4

Synthesis of hepatitic C virus protein fragments and evaluation of their immunogenicity

Karawajczyk B., Maćkiewicz Z., Kunikowska D., Dziadziuszko H., Dera-Tomaszewska B., Głośnicka R., Kupryszewski G.

Polish Journal of Chemistry

|

1998

|

Vol. 72, nr 1

84-88

EN

Three polypeptide fragments of Hepatitis C virus protein (130-140)C, (133-142)C and (1406-1415)NS3 were synthesized using the solid-phase method. The immunogenicity of the peptides was tested on rabbits. All the peptides studied revealed homoral and cell response.

5

Synthesis of flagellin fragments and studies of their interactions with antibodies. Part 1

Klugmann K., Kunikowska D., Głośnicka R., Maćkiewcz Z.

Polish Journal of Chemistry

|

1998

|

Vol. 72, nr 9

2093-2097

EN

Three polypeptide fragments of flagellin protein were synthesized using the solid-phase method. The immunogenicity of the peptides was tested. All the peptides exhibit high immunonological activity.