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1 Acta of Bioengineering and Biomechanics

1 2003

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Cell deformation in response to long-term hyperosmotic loading

Van Donkelaar C.C., Daniels M.A., Huyghe J.M.R.J.

Acta of Bioengineering and Biomechanics

2003

Vol. 5, nr 1

3-10

Hypertrophic and elongated cells are found in differentiation zones of load-bearing tissues, where tissue hyperosmotic. In this paper we study if, in response to long-term hyperosmotic loading, cells are -affected with hypertrophy or elongation, and whether these responses are cell-specific. Surface adhesion and elongation of CHO-K1 and C2C12 cells were determined with CLSM, after 2 and 5 days of culture in 380 mOsmol medium. Results show that both cell types increase an adhesion area (p < 0.001 for CHO-K1 cells, p < 0.01 for C2Cl2 cells), independent of the method used to increase osmotic pressure. Despite the differences :dl types (CHO-K1 cells are smaller (p < 0.001) and their morphological changes are more pronounced). aspect ratio remains constant for all cell types and experimental conditions (p > 0.1). Conclusively, all cells hypertrophy, but do not elongate under hyperosmotic loading. Quantitatively, CHO-K1 cells respond more than C2C12 cells.