Wyniki wyszukiwania - BazTech

1

Miniaturized device for a cell lysis process

Kwapiszewski R., Chudy M., Dybko A., Brzózka Z.

Challenges of Modern Technology

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2011

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Vol. 2, no. 2

50--52

EN

Single-cell studies are crucial for gaining knowledge on complexity of intracellular processes. In many cases, carrying researches into cell ingredients must be proceeded by a lysis process. Cell lysis leads to disintegration of the plasma membrane which is the barrier separating cell contents from the environment. However, investigations at the cellular level would not be possible without proper miniaturized tools, which offer many advantages as low reagents consumption, short reaction time, integration, automation or versatility. The goal of this work was to design and develop a microfluidic chip for a chemical cell lysis process. The geometry of a microsystem presented is based on the hydrodynamic focusing of a cell suspension stream. Applying non-denaturing cell lysis buffer enables to analyze released cell ingredients during next steps of investigations.

2

Modelowanie numeryczne ogniskowania strugi cieczy w węźle mikrokanałów

Domagalski P., Dziubiński M.

Inżynieria i Aparatura Chemiczna

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2009

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Nr 6

43-44

PL

W pracy przedstawiono wyniki symulacji numerycznych procesu ogniskowania strugi cieczy w węźle mikrokanałów. Modelowanymi wielkościami były profile prędkości oraz geometria ogniskowanej strugi. Dostępne dane doświadczalne potwierdzają uzyskane wyniki symulacji.

EN

Results of numerical simulations of hydrodynamic focusing of liquid streams in microchannels are presented in the paper. Velocity profiles and geometry of focusing streams were taken as modelling parameters. Available experimental data confirmed the results of CFD simulations.

3

Hydrodynamic focusing inside rectangular microchannels

Dziubiński M., Domagalski P.

Chemical and Process Engineering

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2007

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Vol. 28, z. 3

567-577

PL

Przedstawiono istniejące możliwości zastosowania techniki hydrodynamicznego ogniskowania strugi cieczy w mikrokanałach, a także wyniki najnowszych badań własnych, które pokazują złożoność hydrodynamiki ogniskowania strugi cieczy w mikrokanałach. Wskazano na nowe, obiecujące kierunki rozwoju tej techniki i jej zastosowania.

EN

The paper presents a review of existing applications of hydrodynamic focusing and the latest research in this area. Hydrodynamic focusing, being a well established technique in microfluidic area has found many applications. The latest researches, however, show precisely a new, complicated three-dimensional aspect of this phenomenon indicating novel, promising possibilities of future applications and development.

4

X-ray microdiffraction on flow-controlled biomolecular assemblies

Evans H.M., Dootz R., Köster S., Pfohl T.

Bulletin of the Polish Academy of Sciences. Technical Sciences

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2007

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Vol. 55, nr 2

217-227

EN

The study of liquid crystalline assemblies, with an emphasis on biological phenomena, is now accessible using newly developed microdevices integrated with X-ray analysis capability. Many biological systems can be described in terms of gradients, mixing, and confinement, all of which can be mimicked with the use of appropriate microfluidic designs. The use of hydro-dynamic focusing creates well-defined mixing conditions that vary depending on parameters such as device geometry, and can be quantified with finite element modelling. We describe experiments in which geometry and strain rate induce finite changes in liquid crystalline orientation. We also demonstrate the online supramolecular assembly of lipoplexes. The measurement of lipoplex orientation as a function of flow velocity allows us to record a relaxation process of the lipoplexes, as evidenced by a remarkable 4-fold azimuthal symmetry. All of these processes ale accessible due to the intentional integration of design elements in the microdevices.

5

Charakterystyka i zastosowanie hydrodynamicznego ogniskowania strugi cieczy w mikrokanałach

Domagalski P., Dziubiński M., Mielnik M. M., Saetran L. R.

Inżynieria i Aparatura Chemiczna

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2006

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Nr 6s

53-54

EN

The article presents characteristics of the hydrodynamical focusing accompanied by a review of possible applications. The dependence of the focused sheet position on side stream velocities and the dependence of the sheet curvature on its position in the outlet channel are presented and discussed. The results enable one to conclude that the application of hydrodynamical focusing in reaction engineering is highly profitable.