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GC-MS Analysis of the Essential Oil from Flowers of Chrysanthemum coronarium L. Propagated Conventionally and Derived from In Vitro Cultures

Wesołowska A., Grzeszczuk M., Kulpa D.

Acta Chromatographica

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2015

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Vol. 27, no. 3

525--539

EN

The composition of the essential oils obtained by hydrodistillation from flowers of Chrysanthemum coronarium L. has been investigated by gas chromatography-mass spectrometry (GC-MS). A total of 105 different constituents were identified, representing an average of 97.71–99.95% of the total oil composition. The oil obtained from flowers propagated conventionally contained the higher amounts of cis-chrysanthemol, cischrysanthenyl isovalerate, camphor, and virdiflorol, while the higher concentration of bornyl acetate, cis-chrysanthenyl acetate, and 2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4.4] non-3-ene was noted in the oil obtained from flowers propagated in vitro. Significantly higher amounts of essential oil were obtained from flowers of plants derived for in vitro propagation (0.58%) in comparison with conventional cultivation (0.50%).

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GC-MS analysis and comparison of volatile compounds of Salvia aucheri Boiss. var. mesatlantica Maire., obtained by hydrodistillation and headspace solid phase microextraction (HS-SPME)

Znini M., Majidi L., Desjobert J. M., Paolini J., Costa J.

Acta Chromatographica

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2014

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Vol. 26, no. 3

495--505

EN

The essential oil and the volatile compounds of Salvia aucheri Boiss. var. mesatlantica Maire were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). The volatile compounds were detected using headspace-solid phase microextraction (HS-SPME) under optimized parameters. A comparative qualitative and quantitative study on the composition of the oils was carried out. For this, 38 compounds, constituting 95.40% of the oil, were identified in the essential oil by hydrodistillation (HD), and 32 compounds, representing 93% of the oil, were characterized by HS-SPME. The major components identified are camphor (49.80%, 51.80%), 1,8-cineole (9.50%, 9.40%), viridiflorol (8.80%, 1.40%), camphene (7.80%, 10.60%), α-pinene (2.90%, 4.50%), and p-cymene (1.50%, 2.40%) of essential oil and volatile compounds detected in HS-SPME, respectively. Quantitative but not qualitative differences have been found in the chemical composition of both analyzed samples depending on the extraction method. For these reasons, HS-SPME can be considered as an alternative technique for isolating volatiles from aromatic plants.

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Chemical components and antidiabetic activity of essential oils obtained by hydrodistillation and three solvent extraction methods from Carthamus tinctorius L.

Li L., Wang Q., Yang Y., Wu G, Xin X., Aisa H. A

Acta Chromatographica

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2012

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Vol. 24, no. 4

653--665

EN

The chemical compositions of essential oils extracted by n-hexane extract (HE), petroleum ether extract (PE), dichloromethane extract (DE), and hydrodistillation (HD) from Carthamus tinctorius L. (safflower) were analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 86 compounds from four different extracts were identified, and the contents were 97.65%, 98.05%, 98.93%, and 99.68%, respectively. 6,10,14-Trimethyl-2-pentadecanone, hexadecanoic acid, methyl ester, hexadecanoic acid, 8,11-octadecadienoic acid, methyl ester, and 9,12,15-octadecatrien-1-ol were the major constituents of the extracts. The antidiabete activity was assayed in vitro by against protein tyrosine phosphatase 1B (PTP1B). The results showed that the HE exhibited the best in vitro inhibitory enzyme activity against PTP1B, which holds a good potential for treating diabetes and obesity.

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Analysis of the volatile compounds in Nepeta crispa willd. Using improved HS-SPME-GC-MS and comparison with conventional methods

Azar P. A, Porgham-Daryasari A, Saber-Tehrani M, Soleimani M.

Acta Chromatographica

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2012

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Vol. 24, no. 1

75--84

EN

In this study, gas chromatography-mass spectrometry (GC-MS) following microwave distillation and solid-phase microextraction (MD-SPME) was developed for the analysis of essential oil compounds in Nepeta crispa. To improve the headspace (HS) method, microwave powers, irradiation times, and SPME fiber coatings were studied. The optimal experiment parameters obtained were 65-μm PDMS/DVB SPME fiber, a microwave power of 400 W, and an irradiation time of 3 min. MD-SPME was compared with headspace solid-phase microextraction (HS-SPME) and conventional hydrodistillation (HD) for the extraction of essential oil compounds in N. crispa. A comparative qualitative and quantitative study on the composition of the oils was carried out. For this, 26 compounds, constituting 98.2% of the oil, were identified in the essential oil by MD-SPME, and 23 compounds, representing 91.3% of the oil, were characterized in the SPME and HD methods; 25 compounds, constituting 94.6% of the oil, were separated and identified. The main compounds identified by all methods were 1,8-cineol, β-bourbonene, α-terpineol, and β-pinene. The relative standard deviation (RSD) values of less than 8% show that the MD-SPME method has good repeatability.It has been shown that the extraction of essential oils from N. crispa with MD-SPME was better in terms of energy saving, extraction time, plant material, oxygenated fractions, and product quality.

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GC-MS analysis of lemon catnip (Nepeta cataria L. var. citriodora Balbis) essential oil

Wesołowska A., Jadczak D, Grzeszczuk M.

Acta Chromatographica

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2011

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Vol. 23, no. 1

169--180

EN

The essential oil of the herb Nepeta cataria L. var. citriodora was obtained by hydrodistillation in Dean-Stark apparatus and by steam distillation. The chemical composition of the oil was determined by GC-MS. Two different GC oven temperature programs were tested. The main constituents of all the samples studied were the monoterpene alcohols (R)-(+)-β-citronellol and geraniol. The largest amounts of monoterpene aldehydes (geranial and neral) were found in the hydrodistilled oil. α-Amyrin (a precursor of ursolic acid) was also detected in the oil. trans-Rose oxide, α-terpineol, geranic acid, and trace amount of rose-oxide were found in the steam distilled oil only.