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Determination of hydrocortisone release profile from polymeric nanocarriers

Miastkowska Małgorzata, Bialik-Wąs Katarzyna, Sycz Anna

Engineering of Biomaterials

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2021

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Vol. 24, no. 163

77

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Preparation and characterization of bio-hybrid hydrogel materials

Malina Dagmara, Królicka Ewelina, Bialik-Wąs Katarzyna, Pluta Klaudia

Engineering of Biomaterials

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2020

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Vol. 23, no. 155

12--16

EN

In recent decades, research has focused on the development of modern hydrogel dressings due to their open porous structure, moisture retention and good mechanical strength, which ensures an optimal environment for cell migration and proliferation. Active hydrogel dressings, currently available on the market, are not endowed with additional medicinal substances. In this work the authors attempted to introduce a carrier-drug system into the hydrogel matrix to improve the wound healing process and the tissue recovery. The main goal of the research was to obtain the bio-hybrid sodium alginate/poly(vinyl alcohol)/Aloe vera (SA/PVA/AV)-based hydrogel matrices modified with the thermosensitive polymeric carrier – the active substance (hydrocortisone) system. First, thermosensitive polymeric nanocarriers were obtained, then the encapsulation was conducted, using varied amounts of hydrocortisone (25 and 50 mg) to maintain the stability of the resulting emulsions. The last stage was preparing the bio-hybrid hydrogel matrices by the chemical cross-linking method. The non-invasive dynamic light scattering (DLS) technique was employed for the analysis of the average particle size of the polymeric carriers and the carrier-drug systems. Moreover, the studies also determined the swelling behaviour and the gel fraction of the obtained bio-hybrid hydrogel matrices modified with carrier-drug systems by the infrared spectroscopy (FT-IR). The presented research results constitute a good experimental basis for further modifications, the final effect of which is assumed to be a modern bio-hybrid 3rd generation dressing.

3

Comparative study of RP-HPLC versus TLC-spectrodensitometric methods applied for binary mixtures of fluoroquinolones and corticosteroids

Elgizawy S. M., Hassan N. Y., Lotfy H. M., Saleh S. S.

Acta Chromatographica

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2014

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Vol. 26, no. 3

439--456

EN

Reversed phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC)-spectrodensitometric methods have been developed and validated for the separation and quantitation of two binary mixtures: Ofloxacin (OFX) and dexamethasone (DXM) in eye preparation; ciprofloxacin hydrochloride (CIP) and hydrocortisone (HYD) in ear preparation. The linearity ranges of RP-HPLC methods were found to be (2.5–45 μg mL-1) for OFX, (2.5–50 μg mL-1) for DXM and (1–8 μg mL-1) for both CIP and HYD. The percentage recoveries/relative standard deviation (RSD) were found to be 100.36/1.38, 100.13/1.49, 99.98/0.61 and 100.28/1.27, respectively. The linearity ranges of TLC-spectrodensitometric methods were found to be (0.5–2 μg band-1), (0.5–3.5 μg band-1), (0.2–1.6 μg band-1), and (0.6–2 μg band-1 for OFX, DXM, CIP, and HYD, respectively. The percentage recoveries/RSD were found to be 99.98/1.06, 99.93/1.18, 99.74/1.27, and 99.94/1.54, respectively. A comparative study was conducted to show the advantages of the proposed methods which showed that the TLC-spectrodensitometric methods were simpler, more sensitive, and economic, while RP-HPLC methods were more precise and robust. The methods were validated in compliance with the ICH guidelines and were successfully applied for determination of the selected drugs in their laboratory-prepared mixtures and commercial dosage forms.

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RP-HPLC procedure for determination of hydrocortisone in serum for use in pharmacokinetics

Główka F. K., Hermann T. W.

Chemia Analityczna

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1999

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Vol. 44, No. 3A

373-380

EN

An RP-HPLC assay to quantify hydrocortione in human serum after prior methylene chloride extraction has been developed for use in pharmacokinetic and/or bioavailability studies. The assay is simple, precise, accurate and sufficiently sensitive Methylprednisolone acetate has been proposed as a stable internal standard to increase the reproducibility of the assay. Mean extraction and processing recoveries for hydrocortisone ranged from 80.1 to 84.6%. Coefficient of variations was between 8.3 and I.1 % for concentrations between 0.05 and 6.00 mg 1 (-1) hydrocortisone, respectively Hydrocorti-sone mean concentrations found in human serum after single intramuscular injection of hydrocortisone sodium succinate (250 m g hydrocortisone) were in the range 009-3.11 mg 1(-1) within 0.3-5.0 h elapsed from the administration to healthy volunteers. Biological halt: life time was 0 78 =O.29 h. Peak serum concentrations were reached atO.44=0.18 h.

PL

Opracowano procedurę RP-HPLC, do analizy ilościowej hydrokortyzonu w surowicy krwi człowieka, po uprzedniej ekstrakcji chlorkiem metylenu. Metodę zastosowano do badań dostępności biologicznej u zdrowych ochotników, którym podano jednorazową dawkę bursztynianu sodu hydrokortyzonu. Wzorcem wewnętrznym by I octan metylo-prednizolonu (trwały związek), który dodaje się do surowicy w celu zwiększenia powtarzalności wyników. Metoda jest prosta, precyzyjna, dokładna i dostatecznie czuła