Wyniki wyszukiwania - BazTech

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Growth and osteogenic differentiation of human osteoblast-like cells on nanofibrous scaffolds loaded with diamond nanoparticles: improvement or impairment?

Musilkova J., Stankova L., Potocky S., Kromka A., Stranska D., Bacakova L.

Engineering of Biomaterials

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2016

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Vol. 19, no. 138 spec. iss.

32

2

Fullerene-titanium (C60/Ti) composites cause no DNA damage response in human osteoblast-like MG 63 cells

Kopova I., Bacakova L., Vacik J., Lavrentiev V.

Engineering of Biomaterials

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2010

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Vol. 13, no. 99-101

109--110

EN

Fullerenes (C60) and fullerene-based composites are considered as promising substrates for biological cell colonization. It might be mainly due to their nanostructure, resembling the nanoarchitecture of the natural extracellular matrix. Thin films of binary C60/Ti composites with various concentrations of Ti ranging from 25% (i.e., 25 Ti atoms and 75 C60 molecules) to 70% were deposited on microscopic glass coverslips in micro-patterned form through a metallic mask, and were tested for their potential use in bone tissue engineering. It is known that fullerenes and their derivatives can cause cytotoxic injury, cell death or inhibition of cell growth. These effects are based mainly on the reactivity of fullerenes, which may weaken with time due to the oxidization and polymerization of fullerenes in an air atmosphere. We therefore tested the dependence between the age of C60/Ti composites (i.e., from one week to one year) and the level of DNA damage of human osteoblast-like MG 63 cells in cultures on these materials. The DNA damage was analyzed by immunofluorescence staining of markers of DNA damage response, such as phosphorylation of histone H2AX and focal recruitment of p53-binding protein. As positive control to markers of DNA damage response was used 7 days long treatment with 2,5 mM Thymidine. We also monitored the proliferation and morphological changes of the cells. After 7 days of cultivation, we observe no cytotoxic morphological changes, such as enlarged cells or cytosolic vacuole formation, which are signs of cell senescence, and no increased induction of cell death. In addition, there was no increased level of DNA damage response on the C60/Ti composites (FIG.1). We also found no significant differences in cell population densities and no increased level of DNA damage among various Ti concentrations (FIG.1). Moreover, there was no effect of the age of the C60/Ti composites on the cell population densities or on the DNA damage response (FIG.1). These results suggest that fullerenes in combination with Ti do not cause cytotoxic injury and this material could be used in bone tissue engineering.

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Pozaustrojowe badania nad toksycznym oddziaływaniem kopolimeru PLGA z dodatkiem hydroksyapatytu na ludzkie osteoblasty linii hFOB 1.19

Cieślik M., Mertas A., Morawska-Chochół A., Orlicki R., Owczarek A., Król W.

Engineering of Biomaterials

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2009

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Vol. 12, no. 89-91

97--102

PL

W celu biologicznej oceny w warunkach in vitro kopolimeru glikolidu z laktydem z dodatkiem hydroksyapatytu (PLGA+HA) dokonano oceny stopnia jego cytotoksyczności względem ludzkich osteoblastów linii hFOB 1.19. Wykonano pomiar aktywności dehydrogenazy mitochondrialnej (test MTT) oraz dehydrogenazy mleczanowej (test LDH) po 24 i 48 godzinach kontaktu komórek z ekstraktem uzyskanym poprzez 8-dniową inkubację kompozytu w medium do hodowli osteoblastów. Kontaktowano ponadto badany materiał bezpośrednio z komórkami kościotwórczymi, a stopień cytotoksyczności oceniano po 24, 48 i 72 godzinach stosując w tym celu test LDH. W obu metodach badany materiał nie wpływał w sposób toksyczny na ludzkie osteoblasty.

EN

In order to evaluate lactide-glycolide co-polymer with admixture of hydroxyapatite (PLGA+HA) from biological point of view in vitro conditions, its level of toxicity for human osteoblasts line hFOB 1.19 was assessed. The activity of mitochondrial dehydrogenase and lactate dehydrogenase was measured (MTT test and LDH test respectively) after 24 and 48 hours of the cells’ contact with the extract obtained through 8-day incubation of the composite in osteoblasts cultivation medium. Apart from that the material examined was contacted with bone-forming cells and its degree of toxicity was assessed after 24, 48 and 72 hours using LDH test. In both methods the material under examination did not have any toxic influence upon human osteoblasts.

4

Morphology expression and proliferation of human osteoblasts on bioactive glass scaffolds

Zhou Z., Chen L.

Materials Science Poland

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2008

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Vol. 26, No. 3

505--516

EN

Bioactive glass was supposed as a biodegradable material and designed as a scaffold to be used for bone reconstruction or regeneration. Bioactive glass scaffold with pore sizes ranged in 100-400µm in diameter was fabricated by sol-gel method, and the biocompatibility evaluation of bioactive glass scaffolds was also performed by culture in vitro models. Cells cultured in the extracts of bioactive glass appeared to show normal morphology. The scaffolds supported osteoblast growth and induced differentiation within the 21 day culture period. Confocal laser scanning microscopy (CLSM) demonstrated the normal cell distribution and proliferation on porous biomaterials. Osteoblasts attached and proliferated on the scaffold as demonstrated by scanning electron microscopy (SEM). Nodule formation and multilayer structures were observed on the scaffold surface and in the pores of the glass. The relationship between seeding density and viability of human osteoblasts cultured on the porous bioactive glass were measured.

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Wpływ wzmocnionego włóknami węglowymi kopolimeru glokolidu z laktydem na odpowiedź komórkową

Cieślik M., Król W., Mertas A., Morawska-Chochół A., Ziąbka M., Chłopek J.

Engineering of Biomaterials

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2008

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Vol. 11, no. 81-84

40--44

PL

W pracy dokonano oceny cytotoksycznego wpływu wzmocnionego włóknami węglowymi kopolimeru glikolidu z laktydem (PLGA+CF) na ludzkie osteoblasty linii hFOB 1.19. Przeprowadzono w tym celu pomiar aktywności dehydrogenazy mitochondrialnej metodą MTT oraz dehydrogenazy mleczanowej (test LDH) w warunkach in vitro. Oba testy nie wykazały toksycznego działania badanego kompozytu na ludzkie komórki kościotwórcze.

EN

This work evaluates the cytotoxic impact of poly-lactide-co-glycolide reinforced with carbon fibres (PLGA+CF) on the hFOB 1.19 human osteoblastic cell line. To this end the levels of miochondrial dehydrogenase (MTT method) and lactate dehydrogenase (LDH test) were measured in vitro. Neither test showed a toxic effect of the studied composite on the human osteogenic cells.

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Ocena cytotoksyczności kopolimeru glikolidu z laktydem (PLGA) w warunkach in vitro

Cieślik M., Król W., Mertas A., Morawska-Chochół A., Ziąbka M., Chłopek J.

Engineering of Biomaterials

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2008

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Vol. 11, no. 81-84

35--39

PL

Celem pracy była ocena in vitro cytotoksycznego działania bioresorbowalnego kopolimeru glikolidu z laktydem (PLGA) na ludzkie osteoblasty linii hFOB 1.19 poprzez pomiar aktywności dehydrogenazy mitochondrialnej (test MTT) oraz dehydrogenazy mleczanowej (test LDH). Do badań użyto ekstrakt uzyskany po 8 dniach inkubacji kopolimeru PLGA w medium wykorzystywanym do hodowli osteoblastów. Ekstrakt ten następnie kontaktowano przez 24 oraz 48 godziny z zaadherowanymi do dna naczynia hodowlanego osteoblastami. Po upływie założonego czasu inkubacji zarówno test MTT, jak i test LDH nie wykazał cytotoksycznego działania kopolimeru PLGA na ludzkie komórki kościotwórcze.

EN

The aim of the work was to evaluate in vitro the cytotoxic effect of bioresorbable polylactide-co-glycolide (PLGA) on the hFOB 1.19 human osteoblastic cell line by measuring the activity of mitochondrial dehydrogenase (MTT test) and lactate dehydrogenase (LDH test). The research made use of an extract obtained after 8 days of PLGA incubation in a medium used for osteoblast culturing. The extract was then brought into contact with osteoblasts adhered to the bottom of the culture vessel for 24 and 48 hours. After the set incubation time neither the MTT test nor the LDH test showed a cytotoxic effect of PLGA on human osteogenic cells.

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Połączenie dwóch metod testowania biomateriałów in vitro

Kudelska-Mazur D., Lewandowska-Szumieł M., Komender J., Benke G.

Inżynieria Biomateriałów

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2000

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R. 3, nr 9

26--29

PL

W pracy przedstawiono połączenie dwóch metod testowania biomateriałów w układzie in vitro. W doświadczeniu wykorzystano osteoblasty i fibroblasty ludzkie otrzymane z hodowli pierwotnych. Komórki wysiano na powierzchnię próbek materiałów: hydroksyapatytu, tlenku glinu i stali chirurgicznej. Zbadano żywotność, liczbę i rozpłaszczenie komórek. Żywotność oznaczono w teście XTT (EL/SA). Komórki poddano znakowaniu barwnikiem fluorescencyjnym Hoechst oraz barwieniu z użyciem przeciwciał anty-a-tubulinie metodą pośrednią. Następnie próbki obserwowano w mikroskopie fluorescencyjnym. Liczbę i rozpłaszczenie komórek zmierzono za pomocą programu do analizy obrazu Image- ProPlus. Wyniki testów żywotności porównano z wynikami pomiarów rozpłaszczenia i liczby komórek. Zauważono korelację pomiędzy rozpłaszczeniem i żywotnością osteoblastów i fibroblastów hodowanych na hydroksyapatycie, tlenku glinu i stali chirurgicznej.

EN

We present a combination of two methods of biomaterial testing in vitro. Primary culture human osteoblasts and fibroblasts seeded on the samples of hydroxyapatite, alumina and surgical steel were used in the experiments. Celt viability, number and spreading were investigated. Viability was estimated in XTT test (ELISA). Anti-a-tubulin antibodies were used for indirect immunofluorescent labelling of cells and Hoechst fluorescent dye for staining of nuclei. Subsequently the samples were observed in a fluorescence microscope. Cell number and spreading were measured by means of Image ProPtus software. The results of viability test were compared with calculations of cell number and spreading, It was observed that correlation existed between spreading and viability of osteoblasts and fibroblasts cultured on hydroxyapatite, alumina and surgical steel.