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1 Polish Journal of Chemistry

1 1998

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Synthesis of human heat shock protein 70 (29-42) fragment and evaluation of its immunogenicity

Karawajczyk B., Wysocki J., Kunikowska D., Maćkiewicz Z., Głośnicka R., Korzeniowski A., Górski J., Kupryszewski G.

Polish Journal of Chemistry

1998

Vol. 72, nr 6

1017-1020

The 29-42 fragment of human heat shock protein 70 (hsp 70) was synthesized by the solid phase method. As determined by enzyme-linked immunosorbent assay (ELISA), the synthetic hsp 70(29-42) peptide generated relatively strong immune response in immunized rabbits. Antibody titers were comparable with anti hsp 70 antibody serum level that was induced by immunization with recombinant protein (hsp 70). It was established that antibodies directed against hsp 70(29-42) peptide could be applied in ELISA for detecting hsp 70 in body fluids and tissues.