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Variations in nutrient concentrations of triticale and barley at different growth stages

Akgün İ., Altındal D.

Infrastruktura i Ekologia Terenów Wiejskich

2017

nr III/1

1061--1073

The objective of this research was to examine the concentrations of crude protein, P, K, Mg, Fe, Mn and Zn of 5 triticale genotypes and 2 barley cultivars (two-row) at different growth stages. The experiments were carried out at Süleyman Demirel University farm in Isparta during the growing season of 2012-2013. Three hexaploid triticale lines (SDÜ- 21, SDÜ-27, SDÜ-43) and 2 cultivars (Karma-2000 and Tatlıcak-97), and two-row barley cultivars (Hamidiye and Cumhuriyet) were used in the experiment. The experimental design was a randomized split block design with three replication. The genotypes were used as main plots and growth stage were used as sub-plots. The basic pre-sowing fertilization rates for all plots were 30 kg N·ha-1 and 50 kg P·ha-1, the rest of 30 kg N·ha-1 was applied at the early spring (stem-elongation stage). Plants were harvested at four stages, stem elongation, milk development, dough development and mature stage. Samples taken from each plot were dried to constant weight at 65°C in oven. After cooling, the samples were milled for crude protein and mineral element analyses. According to the results of variance analysis, the nutrient concentrations of triticale and barley genotypes showed variations depending on the genotypes and different growth stages. The crude protein content of barley cultivars were higher than triticale genotypes. The concentration of K, Fe, Mn and Zn in whole plants decreased from stem elongation to maturity, while Mg and P contents increased. Crude protein rate (18.59%) at dough development stage was higher than other growth stages. The nitrogen use efficiency of SDÜ-27 line, which can be used for cultivar registration, was higher than control cultivar (Karma - 2000; Tatlıcak-1997).

First detailed quantification of silymarin components in the leaves of Silybum marianum cultivated in egypt during different growth stages

Omar A. A, Hadad G. M, Badr J. M

Acta Chromatographica

2012

Vol. 24, no. 3

463--473

A high performance liquid chromatographic method was developed for the quantitative determination of the active components of silymarin in the leaves of Silybum marianum during different growth stages. In this study, taxifolin and six main active constituents in silymarin, including silydianin, silychristin, diastereomers of silybin (silybin A and B) and diastereomers of isosilybin (isosilybin A and B) were completely separated on a 5-μm ODS column (Luna, Phenomenex, USA) with a mobile phase consisting of methanol and 5 mM NaH2PO4 (pH 3.5 adjusted with phosphoric acid) in a ratio of 45:55 v/v. Quantitation was performed with UV detection at 280 nm, based on peak area. The concentration of each component, as well as the total silymarin concentration was determined and compared with those of the seeds, aiming at optimizing the utilization of the cultivated plant. The developed method was validated with respect to linearity, range, specificity, accuracy, precision, and robustness. The leaves were found to contain such concentrations of silymarin components that the yield is better per field area than that from the seeds. Moreover, the extraction of these components from leaves is nonexpensive and simpler than the extraction from seeds.