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Eco friendly synthesis of silver oxide nanoparticles from borassus flabellifer fiber and its antibacterial activity against representative micro organisms

Vanitha R., Kavitha C., Ananda Kumar S.

Advances in Materials Science

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2023

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Vol. 23, nr 4(78)

27--36

EN

The present study reports an easy eco-friendly, cost efficient, and rapid method for the synthesis of silver nanoparticles (Ag NPs) using palm sprouts as reducing cum capping agent. Green synthesis of silver nanoparticles was successfully performed using palm sprouts plant extract via a simple and cheaper eco-friendly method. Palm sprouts extract reduces silver nitrate to silver nanoparticles. The resulting materials were analyzed by Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray Diffraction (XRD) analysis. FT-IR spectrum confirms the presence of various functional groups in the active biomolecules, it acts as a capping agent for the nanoparticles. The morphology of this sample was analyzed through SEM and the presence of silver was confirmed accordingly. The green synthesized Ag NPs exhibited an excellent antibacterial activity against E. coli and P. aeruginosa and B. subtilis and S. aureus besides imparting efficient antimicrobial activity against pathogenic bacteria as well.

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Gentamicin loaded PLGA nanoparticles as local drug delivery system for the osteomyelitis treatment

Posadowska U., Brzychczy-Włoch M., Pamuła E.

Acta of Bioengineering and Biomechanics

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2015

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Vol. 17, no. 3

41--48

EN

Since there are more and more cases of multiresistance among microorganisms, rational use of antibiotics (especially their systemic vs. local application) is of great importance. Here we propose polymeric nanoparticles as locally applied gentamicin delivery system useful in osteomyelitis therapy. Gentamicin sulphate (GS) was encapsulated in the poly(lactide-co-glycolide) (PLGA 85:15) nanoparticles by double emulsification (water/oil/water, W1/O/W2). The nanoparticles were characterized by dynamic light scattering, laser electrophoresis and atomic force microscopy. UV-vis spectroscopy (O-phthaldialdehyde assay, OPA) and Kirby-Bauer tests were used to evaluate drug release and antimicrobial activity, respectively. Physicochemical characterization showed that size, shape and drug solubilization of the nanoparticles mainly depended on GS content and concentration of surface stabilizer (polyvinyl alcohol, PVA). Laser electrophoresis demonstrated negative value of zeta potential of the nanoparticles attributed to PLGA carboxyl end group presence. Drug release studies showed initial burst release followed by prolonged 35-day sustained gentamicin delivery. Agar-diffusion tests performed with pathogens causing osteomyelitis (Staphylococcus aureus and Staphylococcus epidermidis, both reference strains and clinical isolates) showed antibacterial activity of GS loaded nanoparticles (GS-NPs). It can be concluded that GS-NPs are a promising form of biomaterials useful in osteomyelitis therapy.

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Surface properties and antimicrobial activity of composite nanofibers of polycaprolactone with silver precipitations

Dobrzański L., Hudecki A., Chladek G., Król W., Mertas A.

Archives of Materials Science and Engineering

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2014

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Vol. 70, nr 2

53--60

EN

Purpose: The purpose of the article is to investigate the structure and antimicrobial properties of composite nanofibers with silver particles precipitated onto the nanofibers surface. Design/methodology/approach: A solution was prepared in the first place made of the following solvents to fabricate antimicrobial composite nanofibers of polycaprolactone with silver precipitations: formic acid and acetic acid at a rate of 70:30. Then, silver nitrate was introduced into the fabricated solution of the solvents and it was subjected to the interaction of ultrasounds, and after 10 minutes polycaprolactone was added to the solution, and then the solution was mixed for 12 hours and a solution was obtained with a 10% concentration and the mass fraction of 0, 1, 3 and 5% of silver nitrate additives. The solution was forced into a positive voltage electrode placed above a negative voltage electrode; the solution was then subjected to the activity of a strong electrostatic field transforming the solution into micro- and nanofibers. After electrospinning, the fibers obtained underwent the activity of a 2% ascorbic acid solution, by means of which silver was precipitated on the nanofibers surface. Viscosity and electrical conductivity tests were performed of single-component and double-component solutions, of the fibers’ structure in a transmission electron microscope, of the BET, Langmuir specific surface area and DTF porosity with the method of gas adsorption and antimicrobial activity of the nanocomposites produced on the nanocomposites on following bacteria: Staphylococcus aureus, Escherichia coli, Candida albicans. Findings: The use of a formic acid and acetic acid solution at a rate of 70:30 for preparation of a polycaprolactone solution for its electrospinning enables to obtain a non-toxic and fully biodegradable polymer nanofibers of polycaprolactone with PCL/Ag silver precipitations possessing high antimicrobial performance against Gram+, Gram- bacteria and fungi. Practical implications: Antimicrobial composite nanofibers with silver nanoparticles precipitated onto the nanofibers surface can be applied in biodegradable antiseptic dressings in the form of mats or other textiles containing polymer nanofibers of polycaprolactone with PCL/Ag silver precipitations obtained as a result of electrospinning. Originality/value: The research outcomes confirm that it is feasible to manufacture polycaprolactone nanofibers with PCL/Ag silver precipitations possessing high antimicrobial performance against Gram+, Gram- bacteria and fungi.

4

Antybakteryjne działanie żelu bogatopłytkowego - badania in vitro

Bielecki T., Gaździk T.S., Ziółkowski G., Pomorska-Wesołowska M.

Inżynieria Biomateriałów

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2006

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R. 9, nr 54-55

46-49

PL

Mnogość substancji znajdująca się w płytkach, które biorą udział w procesie krzepnięcia, gojenia tkanek i odpowiedzi immunologicznej świadczy o wielkiej roli tych elementów morfologicznych krwi w fizjologii i patologii człowieka. W dostępnej literaturze nie ma publikacji na temat biomateriałów łączących zdolność pobudzenia procesów gojenia z właściwościami antybakteryjnymi. Celem niniejszej pracy było wykazanie antybakteryjnych właściwości żelu bogatopłytkowego. Po okresie inkubacji odczytano wyniki. Średni obszar zahamowania wzrostu w zależności od rodzaju bakterii wahał się między 6,0 a 20,8 mm. Zastosowanie wyżej wymienionego biomateriału, który posiada właściwości bakteriostatyczne i bakteriobójcze może stać się przełomem w leczeniu infekcji tkanek miękkich i ran pourazowych nie tylko w ortopedii, ale również na oddziałach chirurgii ogólnej, naczyniowej, szczękowo-twarzowej, plastycznej oraz torakochirurgii i neurochirurgii

EN

A lot of biological substances which are contained in platelets take a part in coagulation process, tissues healing and in immunological answer, witnesses about huge role these blood elements in physiology and pathology. In literature there are no publications about biomaterials which are able to stimulate healing process and which have antibacterial features. The aim of this experiment is to examine antimicrobial effects of platelet gel. During incubation period results were read. Average area of growth restrain in dependence on bacteria kind between 6,0 and 20,8 mm. Using this biomaterial which own bactericidal and bacteriostatic properties can become a breakthrough in treatment tissue infections and posttraumatic wounds not only in orthopaedics but also surgery vascular, thoracic surgery maxillofacial surgery, plastics surgery and neurosurgery words

5

Gdyby Shaw i Fleming żyli dzisiaj...

Ochocka B.

Laboratorium - Przegląd Ogólnopolski

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2002

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nr 4

16

PL

Wśród gronkowców złocistych coraz większy odsetek stanowią szczepy odporne na metycylinę - MRSA. Pojęcie oporności na metycylinę zawiera w sobie informację, że bakteria ta jest oporna na lecznie większością znanych antybiotyków. Badania wykazują, że MRSA ma także coraz większy udział w tzw. zakażeniach pozaszpitalnych oraz że bakteria ta bardzo szybko rozprzstrzenia się w ekosystemie szpitali. Wysoki udział szczepów MRSA (od 12,5% do 50%) stawia Polskę w czołówce państw o wysokim udziale MRSA w zakażeniach szpitalnych.